Low-Dose Lipopolysaccharide Modifies the Production of IL-12 by Dendritic Cells in Response to Various Cytokines

• Saito Yusuke

  Division of Immunobiology, Institute for Genetic Medicine, Hokkaido University

• Yanagawa Yoshiki

  Division of Immunobiology, Institute for Genetic Medicine, Hokkaido University

• Kikuchi Kazuhiro

  Division of Immunobiology, Institute for Genetic Medicine, Hokkaido University

• Iijima Norifumi

  Division of Immunobiology, Institute for Genetic Medicine, Hokkaido University

• Iwabuchi Kazuya

  Division of Immunobiology, Institute for Genetic Medicine, Hokkaido University

• Onoé Kazunori

  Division of Immunobiology, Institute for Genetic Medicine, Hokkaido University

抄録

Dendritic cell (DC) activation is triggered by cytokines, including tumor necrosis factor (TNF)-α, and microbe components, including lipopolysaccharide (LPS). During the initial stage of infection, the microbe components appear to be present at low concentration. To determine the role of low-dose microbe-components in DC activation during the initial stage of infection, we examined the effects of low-dose LPS on cytokine-induced maturation and function of DCs. Low-dose LPS (1 ng/ml) treatment of DCs had only additive effects on the expression of CD86 and major histocompatibility complex class II induced by various cytokines, including interleukin (IL)-1β, TNF-α and interferon (IFN)-γ. IL-1β alone significantly induced IL-12 production in DCs, whereas TNF-α or IFN-γ induced modest levels of IL-12 production. When low-dose LPS (1 ng/ml), which only slightly induced IL-12 production, was added to the culture, only an additive effect was seen on IL-1β-induced IL-12 production. In contrast, low-dose LPS synergistically enhanced TNF-α- or IFN-γ-induced IL-12 production. SB203580, a specific inhibitor of p38 MAPK, markedly inhibited TNF-α- or IFN-γ-induced IL-12-production either in the absence or presence of LPS, but showed only modest effects on IL-1β-induced IL-12-production. These findings suggest that the p38 MAPK pathway is essential for the synergistic IL-12 production induced by TNF-α- or IFN-γ in combination with low-dose LPS in DC.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　ａｎｄ　Ｅｘｐｅｒｉｍｅｎｔａｌ　Ｈｅｍａｔｏｐａｔｈｏｌｏｇｙ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｃｌｉｎｉｃａｌ　ａｎｄ　Ｅｘｐｅｒｉｍｅｎｔａｌ　Ｈｅｍａｔｏｐａｔｈｏｌｏｇｙ 46 (1), 31-36, 2006

  日本リンパ網内系学会

キーワード

• lipopolysaccharide
• IL-12
• dendritic cells
• TNF-α

詳細情報

• CRID: 1390282679678167680
• NII論文ID: 10018113770
• NII書誌ID: AA11556796
• DOI: 10.3960/jslrt.46.31
• ISSN: 18809952
• ISSN: 13464280
• Web Site: https://search.jamas.or.jp/link/ui/2007003889
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可