Development of ELISA Using Recombinant Antigens for Specific Detection of Mouse Parvovirus Infection

• KUNITA Satoshi

  Laboratory Animal Resource Center, University of Tsukuba

• CHAYA Miyuki

  Laboratory Animal Resource Center, University of Tsukuba

• HAGIWARA Kozue

  Laboratory Animal Resource Center, University of Tsukuba

• ISHIDA Tomoko

  ICLAS Monitoring Center, Central Institute for Experimental Animals

• TAKAKURA Akira

  ICLAS Monitoring Center, Central Institute for Experimental Animals

• SUGIMOTO Tatsuya

  Developmental Research Laboratories, Shionogi & Co., Ltd.

• ISEKI Hiroyoshi

  Laboratory Animal Resource Center, University of Tsukuba

• FUKE Kumiko

  Laboratory Animal Resource Center, University of Tsukuba

• SUGIYAMA Fumihiro

  Laboratory Animal Resource Center, University of Tsukuba

• YAGAMI Ken-ichi

  Laboratory Animal Resource Center, University of Tsukuba

Abstract

Nucleotide sequcences of mouse parvovirus (MPV) isolate, named MPV/UT, and mouse minute virus (MMV) were analyzed and used for expressing recombinant proteins in E. coli. ELISA tests using recombinant major capsid protein (rVP2) and recombinant major non-structural protein (rNS1) as antigens were developed and their performance in serologic detection of rodent parvovirus infection was assessed. MPV-rVP2 and MMV-rVP2 ELISAs reacted specifically with anti-MPV and anti-MMV mouse sera, respectively. MMV-rNS1 antigen had a wide reaction range with antisera to rodent parvoviruses including MPV, MMV, Kilham rat virus (KRV) and H-1 virus. All mice oronasally infected with MPV were seropositive at 4 weeks post-infection in screening by ELISAs using MPV-rVP2 and MMV-rNS1 antigens, but were negative by conventional ELISA using whole MMV antigen. A contact transmission experiment revealed that transmission of MPV occurred up to 4 weeks post-infection, and all cage mates were seropositive in screening with MPV-rVP2 and MMV-rNS1 ELISAs. These results indicate that MPV-rVP2 and MMV-rVP2 are specific ELISA antigens which distinguish between MPV and MVM infection, while MMV-rNS1 antigen can be used in generic ELISA for a variety of rodent parvoviruses. The higher sensitivity of MPV-rVP2 ELISA than conventional ELISA for detecting seroconversion to MPV in oronasally infected mice as well as in cage mates suggests the usefulness of MPV-rVP2 ELISA in quarantine and microbiological monitoring of MPV infection in laboratory mice.<br>

Journal

• Experimental Animals

  Experimental Animals 55 (2), 117-124, 2006

  Japanese Association for Laboratory Animal Science

Keywords

• ELISA
• mouse
• parvovirus
• recombinant antigen

Details

• CRID: 1390282680022052096
• NII Article ID: 10018114688
• NII Book ID: AA11032321
• DOI: 10.1538/expanim.55.117
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL BIB ID: 7881115
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7881115
• Web Site: http://www.jstage.jst.go.jp/article/expanim/55/2/55_2_117/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007066282
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed