C57BL/6J遺伝的背景の高効率FLPeディリーターマウス High-efficiency CAG-FLPe Deleter Mice in C57BL/6J Background

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著者

    • 神吉 浩明 KANKI Hiroaki
    • Laboratory for Behavioral Genetics, Brain Science Institute (BSI), The Institute of Physical and Chemical Research (RIKEN)
    • 鈴木 瞳 SUZUKI Hitomi
    • Laboratory for Behavioral Genetics, Brain Science Institute (BSI), The Institute of Physical and Chemical Research (RIKEN)

抄録

The increasing popularity of conditional knockout (KO) technology has resulted in the demand for efficient FLP deleter mice. In addition, FLP deleters are needed in genetic backgrounds that are suited to behavioral studies. We generated <i>CAG-FLPe</i> transgenic (Tg) mice with the C57BL/6J genetic background, which is one of the most commonly-used strains in behavioral studies. We assessed the recombination efficiency of the <i>CAG-FLPe</i>-Tg lines by crossing them with a mouse line carrying a <i>FRT-PGK-neo-FRT</i> cassette. Four of five independent <i>CAG-FLPe</i> lines induced recombination in most (91%-100%) of their progenies, although a small fraction (0%-30%, depending on the line) showed mosaic recombination patterns. These animals are highly potent as deleters of <i>FRT</i> cassettes and are useful for behavioral studies involving conditional KO mice.<br>

収録刊行物

  • Experimental animals  

    Experimental animals 55(2), 137-141, 2006-04-01 

    Japanese Association for Laboratory Animal Science

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各種コード

  • NII論文ID(NAID)
    10018114740
  • NII書誌ID(NCID)
    AA11032321
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13411357
  • NDL 記事登録ID
    7881144
  • NDL 雑誌分類
    ZS7(科学技術--医学)
  • NDL 請求記号
    Z54-H752
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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