High-efficiency CAG-FLPe Deleter Mice in C57BL/6J Background

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  • KANKI Hiroaki
    Laboratory for Behavioral Genetics, Brain Science Institute (BSI), The Institute of Physical and Chemical Research (RIKEN)
  • SUZUKI Hitomi
    Laboratory for Behavioral Genetics, Brain Science Institute (BSI), The Institute of Physical and Chemical Research (RIKEN)
  • ITOHARA Shigeyoshi
    Laboratory for Behavioral Genetics, Brain Science Institute (BSI), The Institute of Physical and Chemical Research (RIKEN)

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  • High-efficiency CAG-FLPe delete mice in C57BL/6J background

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Abstract

The increasing popularity of conditional knockout (KO) technology has resulted in the demand for efficient FLP deleter mice. In addition, FLP deleters are needed in genetic backgrounds that are suited to behavioral studies. We generated CAG-FLPe transgenic (Tg) mice with the C57BL/6J genetic background, which is one of the most commonly-used strains in behavioral studies. We assessed the recombination efficiency of the CAG-FLPe-Tg lines by crossing them with a mouse line carrying a FRT-PGK-neo-FRT cassette. Four of five independent CAG-FLPe lines induced recombination in most (91%-100%) of their progenies, although a small fraction (0%-30%, depending on the line) showed mosaic recombination patterns. These animals are highly potent as deleters of FRT cassettes and are useful for behavioral studies involving conditional KO mice.<br>

Journal

  • Experimental Animals

    Experimental Animals 55 (2), 137-141, 2006

    Japanese Association for Laboratory Animal Science

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