Changes in Submaxillary Gland Gene Expression in F344 Rats by Multiple Dosing of Theophylline

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  • KAJIKAWA Satoru
    Drug Safety Research Laboratories, Astellas Pharma Inc.
  • KIGAMI Daisuke
    Drug Safety Research Laboratories, Astellas Pharma Inc.
  • NAKAYAMA Hiroyuki
    Department of Veterinary Pathology, Graduate School of Agricultural and Life Science, The University of Tokyo
  • DOI Kunio
    Department of Veterinary Pathology, Graduate School of Agricultural and Life Science, The University of Tokyo

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Abstract

Multiple exposure to theophylline, a phosphodiesterase (PDE) inhibitor, induces acinar hypertrophy in the salivary gland. This study examined the effect of theophylline on the gene expression of secretory proteins and phosphodiesterases in the submaxillary gland. Male F344 rats received saline or theophylline (50 mg/kg) intraperitoneally for 4 days. The gene expressions for the secretory protein, cystatin S (CysS), and PDE subfamilies 3A and 4D in the submaxillary gland were quantified using RT-PCR. Theophylline exposure resulted in a sustained increase in mRNA expression for CysS and PDE3A, but PDE4D gene expression was unchanged. Our results suggest that submaxillary hypertrophy is primarily caused by the enhanced transcription of CysS, and that the transcription of each PDE subfamily gene is regulated differently.<br>

Journal

  • Experimental Animals

    Experimental Animals 55 (2), 143-146, 2006

    Japanese Association for Laboratory Animal Science

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