Requirement of a bone morphogenetic protein for the maintenance and stimulation of osteoblast differentiation
-
- Martinovic Snjezana
- Department of Anatomy, Zagreb Medical School
-
- Borovecki Fran
- Department of Anatomy, Zagreb Medical School
-
- Miljavac Vera
- Department of Anatomy, Zagreb Medical School
-
- Kisic Veronika
- Department of Anatomy, Zagreb Medical School
-
- Maticic Drazen
- Department of Surgery, Zagreb School of Veterinary Medicine
-
- Francetic Igor
- Department of Medicine, Clinical Hospital Center Zagreb
-
- Vukicevic Slobodan
- Department of Anatomy, Zagreb Medical School
Search this article
Abstract
The requirement of a bone morphogenetic protein for the maintenance and stimulation of an osteoblast phenotype was examined using mouse MC3T3-E1 cell cultures. Cells expressed BMP-4 mRNA, which correlated with the stimulation of the osteoblast phenotype. The addition of a BMP-4 specific antibody reduced bone nodules, suggesting that BMP-4 is required for the osteogenic activity of osteoblasts in an autocrine manner. Exogenously added BMP-7 gradually decreased the expression of BMP-4 with a concurrent stimulation of the osteoblast phenotype. Exogenous BMP-7 can therefore substitute for endogenously produced BMP-4 acting as a paracrine factor on osteoblasts.The addition of 17β estradiol decreased BMP-4 expression but initiated synthesis of BMP-6 mRNA, an endocrine signal for osteoblasts, which also substituted for the lack of endogenous BMP-4, as evidenced by normal bone nodule formation. The addition of dexamethasone and parathyroid hormone did not affect the BMP-4 expression but induced transcripts for BMP-2 and BMP-3, respectively, suggesting that their effects on bone can be in part achieved via the BMP signaling. These experiments support the requirement of a BMP for osteoblast differentiation and function, demonstrating for the first time that a BMP can functionally substitute for another BMP in an autocrine/paracrine manner or mediate a response to an endocrine action on osteoblasts.
Journal
-
- Archives of Histology and Cytology
-
Archives of Histology and Cytology 69 (1), 23-36, 2006
International Society of Histology and Cytology
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390282681388808576
-
- NII Article ID
- 130004462662
- 10018116912
-
- NII Book ID
- AA1068990X
-
- ISSN
- 13491717
- 09149465
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- Crossref
- CiNii Articles
-
- Abstract License Flag
- Disallowed