Properties of Monolithic Silica Columns for HPLC

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  • KOBAYASHI Hiroshi
    Department of Polymer Science and Engineering, Kyoto Institute of Technology
  • IKEGAMI Tohru
    Department of Polymer Science and Engineering, Kyoto Institute of Technology
  • KIMURA Hiroshi
    Department of Polymer Science and Engineering, Kyoto Institute of Technology
  • HARA Takeshi
    Department of Polymer Science and Engineering, Kyoto Institute of Technology
  • TOKUDA Daisuke
    Department of Polymer Science and Engineering, Kyoto Institute of Technology
  • TANAKA Nobuo
    Department of Polymer Science and Engineering, Kyoto Institute of Technology

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抄録

Monolithic silica columns and their use in high peak-capacity HPLC separations are reviewed. Monolithic silica columns can potentially provide higher overall performance than particle-packed columns based on the variable external porosity and variable through-pore size/skeleton size ratios. The high permeability of monolithic silica columns resulting from the high porosity is shown to be advantageous to generate large numbers of theoretical plates with long capillary columns. High permeability together with the high stability of the network structures of silica allows their use in high-speed separations required for a second-dimension column in two dimensional HPLC. Disadvantages of monolithic silica columns are also described.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 22 (4), 491-501, 2006

    社団法人 日本分析化学会

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