Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials

この論文にアクセスする

この論文をさがす

著者

抄録

A new method for the qualitative analysis of adenosine nucleotides (AMP, ADP, and ATP) and synthetic oligonucleotides has been proposed, utilizing a pH- and temperature-responsive polymer of <i>N</i>-isopropylacrylamide (NIPAAm), butyl methacrylate (BMA) and <i>N</i>,<i>N</i>-dimethylaminopropylacrylamide (DMAPAAm) as the stationary phase of HPLC. In the chromatographic system using the copolymer with ionizable groups of modified packing materials, we investigated how to separate adenosine nucleotides and oligonucleotides by temperature. The properties of the surface of the copolymer-grafted stationary phase altered from hydrophilic to hydrophobic and from charged to non-charged due to changes in the temperature and in the pH, respectively. In addition, it is possible to exhibit and hide ion-exchange groups on the polymer chain surface by temperature changes. These phenomena result from changes in the charge and hydrophobicity of the pH- and temperature-responsive polymer on the stationary surface with the controlling temperature. A pH- and temperature-responsive chromatography would be greatly useful for biopolymer and nucleotide separation and purification.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 22(4), 539-543, 2006-04-10

    The Japan Society for Analytical Chemistry

参考文献:  33件中 1-33件 を表示

  • <no title>

    KOPECEK J.

    J. Polym. Sci., A 1(9), 2801, 1971

    被引用文献1件

  • <no title>

    TANAKA T.

    Science 218, 467, 1981

    被引用文献1件

  • <no title>

    OKANO T.

    J. Biomed. Mater. Res. 27, 1243, 1993

    被引用文献1件

  • <no title>

    SHIMIZU T.

    Tissue Eng. 7, 141, 2001

    被引用文献1件

  • <no title>

    KANAZAWA H.

    Anal. Sci. 18, 45, 2002

    被引用文献1件

  • <no title>

    KANAZAWA H.

    Trends Anal. Chem. 17, 435, 1998

    被引用文献1件

  • <no title>

    SAKAMOTO C.

    J. Chromatogr., A 1030, 247, 2004

    被引用文献1件

  • <no title>

    AYANO E.

    Anal. Sci. 17, i873, 2001

    被引用文献1件

  • <no title>

    YAMAMOTO K.

    Chromatogr. 209, 21, 2000

    被引用文献1件

  • <no title>

    CADET J.

    Anal. Chem. 65, 675, 1993

    被引用文献8件

  • <no title>

    PON R. T.

    Biotechnology 21, 680, 1996

    被引用文献2件

  • <no title>

    OEFINER P. J.

    J. Chromatogr., B 782, 27, 2002

    DOI 被引用文献1件

  • <no title>

    BUCK G. A.

    Biotechniques 27, 528, 1999

    被引用文献1件

  • <no title>

    GILAR M.

    J. Chromatogr., A 890, 167, 2000

    被引用文献1件

  • <no title>

    WARREN W. J.

    Mol. Biotechnol. 4, 179, 1995

    被引用文献1件

  • <no title>

    HORN T.

    Nucleic Acids Res. 16, 11559, 1988

    被引用文献1件

  • <no title>

    HILL T. L.

    J. Chromatogr. 512, 415, 1990

    被引用文献2件

  • <no title>

    GILAR M.

    J. Chromatogr. A 958, 167, 2002

    被引用文献3件

  • <no title>

    HUANG G.

    Anal. Biochem. 190, 21, 1990

    被引用文献2件

  • <no title>

    XU Y.

    Anal. Biochem. 204, 185, 1992

    被引用文献1件

  • <no title>

    BAE Y. H.

    J. Polym. Sci. Polym. Phys. 28, 923, 1990

    DOI 被引用文献22件

  • <no title>

    MATSUKATA M.

    Microporous Mater. 7, 109-117, 1996

    被引用文献6件

  • <no title>

    HESKINS M.

    J. Macromol Sci. Chem. A 2, 1441, 1968

    DOI 被引用文献102件

  • <no title>

    KANAZAWA H.

    Anal. Chem. 68, 100, 1996

    DOI 被引用文献23件

  • <no title>

    KANAZAWA H.

    Anal. Chem. 69, 823, 1997

    DOI 被引用文献13件

  • <no title>

    KANAZAWA H.

    J. Pharm. Biomed. Anal. 15, 1545, 1997

    被引用文献7件

  • <no title>

    YAMAMOTO K.

    Environ. Sci. 7(1), 47, 2000

    被引用文献7件

  • <no title>

    KANAZAWA H.

    Anal. Chem. 72, 5961, 2000

    DOI 被引用文献14件

  • <no title>

    KOBAYASHI J.

    Anal. Chem. 73, 2027, 2001

    DOI 被引用文献8件

  • <no title>

    KOBAYASHI J.

    J. Chromatogr. A 958, 109, 2002

    DOI 被引用文献10件

  • <no title>

    BULMUS V.

    Bioconjugate Chem. 11, 78, 2000

    DOI 被引用文献6件

  • <no title>

    FEIL H.

    Macromolecules 25, 5528, 1992

    被引用文献6件

  • <no title>

    HUBER C. G.

    Anal. Biochem. 212, 351, 1993

    被引用文献1件

被引用文献:  1件中 1-1件 を表示

各種コード

  • NII論文ID(NAID)
    10018123757
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    7871917
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ