Large-Volume Sample Sweeping with a High Theoretical Plate Number Using a Coupled-Capillary in Capillary Electrophoresis
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A large-volume sample injection (> 5 µL) with an extremely high theoretical plate number (<i>N</i> > 10<sup>7</sup>) was achieved when the sweeping-MEKC mode and a coupled-capillary (100 - 50 µm i.d.) were simultaneously used in a capillary electrophoresis (CE) separation. A low-cost and compact violet-LED (∼2 mW) was used as the fluorescence excitation source. As a result, the theoretical plate numbers of the detected peaks (two model compounds: naphthalene-2,3-dicarboxaldehyde derivatized-dopamine and -norepinephrine) were 1.0 × 10<sup>7</sup> and 7.4 × 10<sup>6</sup>, respectively. The limits of detection (at <i>S/N</i> = 3) of these were determined to be 2.8 × 10<sup>-10</sup> M (92 ppt) and 2.3 × 10<sup>-10</sup> M (83 ppt), respectively.
- Analytical Sciences
Analytical Sciences 22(4), 557-561, 2006-04-10
The Japan Society for Analytical Chemistry