Histological and immunohistochemical changes in the submandibular gland in klotho-deficient mice

• Suzuki Hironobu

  Division of Anatomy and Cell Biology of the Hard Tissue, Niigata University Graduate School of Medical and Dental Sciences

• Amizuka Norio

  Center for Transdisciplinary Research, Niigata University

• Noda Masaki

  Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University

• Amano Osamu

  Division of Anatomy, Department of Human Development and Fostering, Meikai University School of Dentistry

• Maeda Takeyasu

  Center for Transdisciplinary Research, Niigata University Division of Oral Anatomy, Department of Oral Biological Science, Niigata University Graduate School of Medical and Dental Sciences

抄録

The submandibular gland (SMG) has been regarded as an age-stable organ in spite of reports on its structural changes with aging. Although the klotho gene is involved in aging, little information is available regarding its effects on morphological changes of SMGs. The present study examined the histological and immunohistochemical features of SMGs in klotho-deficient mice - which are well-established aging models - by immunohistochemical and histochemical techniques. Five kinds of cellular markers - against NGF, EGF, Mnand Cu/Zn-SOD, and RITC-conjugated phalloidin - were used for the identification of cell types. In klotho-deficient mice, the SMGs lost their granular ducts and each lobe diminished. The granular duct showed strong immunoreactivities for NGF and EGF in the wild-type mice, but the NGF- and EGF-immunopositive ducts decreased in number remarkably in klotho-deficient mice. Interestingly, instead of a loss of the granular duct, the striated duct located on the distal portion in the homozygous mice came to show NGF- and EGF-immunoreactions. Neither Mn- and Cu/Zn-SOD immunoreactivities in the duct system nor the phalloidin- reaction in the myoepithelial cells differed between the wild-type and klotho-deficient mice. Our findings suggest that the klotho gene inhibited the differentiation of the granular duct from the striated duct due to the repression and/or down-regulation of sexual and growth hormones.

収録刊行物

• Ａｒｃｈｉｖｅｓ　ｏｆ　Ｈｉｓｔｏｌｏｇｙ　ａｎｄ　Ｃｙｔｏｌｏｇｙ

  Ａｒｃｈｉｖｅｓ　ｏｆ　Ｈｉｓｔｏｌｏｇｙ　ａｎｄ　Ｃｙｔｏｌｏｇｙ 69 (2), 119-128, 2006

  国際組織細胞学会

キーワード

• Histology

詳細情報

• CRID: 1390001206411675136
• NII論文ID: 130004462669
• NII論文ID: 10018151691
• NII書誌ID: AA1068990X
• DOI: 10.1679/aohc.69.119
• ISSN: 13491717
• ISSN: 09149465
• Web Site: http://www.jstage.jst.go.jp/article/aohc/69/2/69_2_119/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007079602
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可