抗ヒトＯＰＲＴウサギポリクローナル抗体を用いた大腸癌におけるＯＰＲＴ蛋白発現の検討

• 山田 正樹

  順天堂大学下部消化管外科

• 瀧田 尚仁

  順天堂大学下部消化管外科

• 北島 政幸

  順天堂大学下部消化管外科

• 笠巻 伸二

  順天堂大学下部消化管外科

• 坂本 一博

  順天堂大学下部消化管外科

• 落合 匠

  東京都保健医療公社東部地域病院外科

• 鎌野 俊紀

  順天堂大学下部消化管外科

書誌事項

タイトル別名

• Assessment of Orotate Phosphoribosyl Transferase Protein Expression using the Anti-human OPRT Rabbit Polyclonal Antibody in Colorectal Cancer

抄録

Relationships among orotate phosphoribosyl transferase (OPRT) protein expression, measured using antihuman OPRT rabbit polyclonal antibody ; OPRT enzyme activity, clinicopathological background factors, and single nucleotide polymorphisms (SNPs) in the OPRT coding region were examined. Thirty-five subjects who had undergone surgical resection of colon cancer in our hospital between December 2003 and July 2004 were enrolled in the study. OPRT activity at the tumor sites was significantly higher than in normal regions. Based on immunohistochemical staining, strong expression of OPRT was noted in 24 subjects. The OPRT activity in these subjects was significantly higher compaired with subjects with low OPRT expression. No relationships were found among OPRT protein expression, clinicopathological background factors, and SNPs in the OPRT gene. These results suggest that OPRT enzyme activity can be predicted using OPRT immunohistochemical staining, and that patients with hypersensitivity to 5-fluorouracil (5-FU) can easily be identified using this method.

収録刊行物

• 日本大腸肛門病学会雑誌

  日本大腸肛門病学会雑誌 59 (3), 113-119, 2006

  日本大腸肛門病学会

キーワード

• 大腸癌
• Orotate Phosphoribosyl Transferase
• 免疫組織化学染色
• single nucleotide polymorphisms

詳細情報

• CRID: 1390282679830732160
• NII論文ID: 10018167538
• NII書誌ID: AN00195100
• DOI: 10.3862/jcoloproctology.59.113
• ISSN: 18829619
• ISSN: 00471801
• Web Site: http://www.jstage.jst.go.jp/article/jcoloproctology1967/59/3/59_3_113/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006113002
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可