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- SUGO Ken
- Research and Development Department, New Ceramics Division, Life Care Headquarters, PENTAX Comoration
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- KATO Machiko
- Research and Development Department, New Ceramics Division, Life Care Headquarters, PENTAX Comoration
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- ISHIKAWA Tsuyoshi
- Research and Development Department, New Ceramics Division, Life Care Headquarters, PENTAX Comoration
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- YAMAMOTO Akira
- Incubation Center, PENTAX Corporation
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- OGAWA Tetsuro
- Research and Development Department, New Ceramics Division, Life Care Headquarters, PENTAX Comoration
この論文をさがす
抄録
We developed and characterized hydroxyapatite (HA) microcarrier for high-density cell culture. HA microcarrier was prepared by coating the surface of polymer (nylon, epoxy and polyethylene)beads with HA particles. The mixtures of polymer beads and HA were processed with a Mechanofusion system. Nylon beads with HA showed the most suitable coating efficiency, and the specific gravity, according to scanning electron microscopy and a gas pycnometer. HA microcarriers with four different composition ratios were prepared from mixtures of nylon beads and HA at weight ratios of 100:0.5, 100:1, 100:2 and 100:3, to determine the optimum mixture ratio. Vero cells were cultured by use of these HA microcarriers. The HA microcarrier with a weight ratio of 100:1 was the most suitable cell carrier bead, because of its specific gravity, and the effective ability for Vero cells to proliferate on it.
収録刊行物
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- TISSUE CULTURE RESEARCH COMMUNICATIONS
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TISSUE CULTURE RESEARCH COMMUNICATIONS 25 (2), 105-111, 2006
The Japanese Tissue Culture Association
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詳細情報 詳細情報について
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- CRID
- 1390001205466811392
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- NII論文ID
- 130004640626
- 10018198151
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- NII書誌ID
- AA11048252
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- ISSN
- 09123636
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可