HYDROXYAPATITE MICROCARRIER (2)
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- SUGO Ken
- Research and Development Department, New Ceramics Division, Life Care Headquarters, PENTAX Corporation
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- KATO Machiko
- Research and Development Department, New Ceramics Division, Life Care Headquarters, PENTAX Corporation
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- ISHIKAWA Tsuyoshi
- Research and Development Department, New Ceramics Division, Life Care Headquarters, PENTAX Corporation
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- YAMAMOTO Akira
- Incubation Center, PENTAX Corporation
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- OGAWA Tetsuro
- Research and Development Department, New Ceramics Division, Life Care Headquarters, PENTAX Corporation
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Abstract
We developed a hydroxyapatite (HA) microcarrier prepared by coating the surface of nylon beads with HA particles. Cell growth ability and viability were observed on the newly produced HA microcarrier (CELLYARD beads) and compared with that on a commercially available dextran microcarrier (Cytodex 1). Madin-Darby canine kidney cells (MDCK) and Vero cells were cultured using CELLYARD beads and Cytodex 1 in a 100 mL spinner flask. At 5days of incubation, MDCK cells proliferated on CELLYARD beads (2.31 × 106 cells/mL) effectively, at about 1.6 times the cell densities for Cytodex 1 (1.44 × 106 cells/mL). Vero cells also proliferated on CELLYARD beads (8.40 × 105 cells/mL) effectively, almost equal to that for Cytodex 1 (8.85 × 105 cells/mL). MDCK cells and Vero cells harvested from CELLYARD beads had much higher viability than those from Cytodex 1. CELLYARD beads had equal or better performance, compared with Cytodex 1, and would be a good material for high-density cell culture.
Journal
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- TISSUE CULTURE RESEARCH COMMUNICATIONS
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TISSUE CULTURE RESEARCH COMMUNICATIONS 25 (2), 113-118, 2006
The Japanese Tissue Culture Association
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Details 詳細情報について
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- CRID
- 1390001205466809728
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- NII Article ID
- 130004640627
- 10018198164
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- NII Book ID
- AA11048252
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- ISSN
- 09123636
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- Text Lang
- en
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed