Crystallization of β-Sitosterol Using a Water-Immiscible Solvent Hexane
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Cooling crystallization of <I>β</I>-sitosterol that is a kind of phytosterol was carried out, where a water-immiscible solvent hexane containing a ppm level of water was used as a solvent. The initial ratio of water and <I>β</I>-sitosterol concentration adopted in this work was 330/10, 1315/10 and 645/20 ppm/(g/L), which corresponded to 0.51, 2.0, and 0.50 in water/<I>β</I>-sitosterol mole ratio, respectively. When the initial ratio of water and <I>β</I>-sitosterol concentration was 1315/10 ppm/(g/L), thin plate-like crystals were precipitated and were identified as the monohydrate crystals of <I>β</I>-sitosterol. On the other hand, when the initial ratio of water and <I>β</I>-sitosterol concentration was 645/20 ppm/(g/L), needle-like crystals of hemihydrate were precipitated. This result meant that the ppm level of water plays an important role for the pseudo-polymorphism. Since the crystal structure of <I>β</I>-sitosterol hemihydrate had never been reported, we determined it by a single crystal XRD analysis.
- JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 39(8), 869-875, 2006-08-01
The Society of Chemical Engineers, Japan