Subcellular Localization of a Germiantion-specific Cortex-lytic Enzyme, SleB, of Bacilli during Sporulation

この論文にアクセスする

この論文をさがす

著者

抄録

The subcellular localization of a germination-specific cortex-lytic enzyme, SleB, of <i>Bacillus subtilis</i> during sporulation was observed by using fusions of N-terminal region of SleB to the green fluorescent protein (GFP). A fusion with a putative peptidoglycan-binding motif (SleB<sub>1-108</sub>-GFP) formed a fluorescent ring around the forespore of the wild type strain, as expected from the known location of the intact SleB in the dormant spore. SleB<sub>1-108</sub>-GFP formed a similar fluorescent ring around the forespore of the <i>gerE</i> mutant which has a severe defect in the coat structure, and of the <i>cwlD</i> mutant which lacks a muramic δ-lactam unique to the spore peptidoglycan (cortex), whereas the fusion could not attach to the spore of the <i>cwlDgerE</i> mutant. By contrast, a fusion without the motif (SleB<sub>1-45</sub>-GFP) could not be recruited around the forespore of the <i>gerE</i> mutant though it appeared to be accumulated on the outside of the spore of the wild type strain. Since SleB was shown to degrade only the cortex with muramic δ-lactam, these results suggested that a proper localization of SleB requires a strict interaction between the motif of the enzyme and the δ-lactam structure of the cortex, not the formation of normal coat layer.<br>

収録刊行物

  • Genes & genetic systems  

    Genes & genetic systems 81(3), 163-169, 2006-06-25 

    The Genetics Society of Japan

参考文献:  28件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

被引用文献:  1件

被引用文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10018212628
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • NDL 記事登録ID
    7997878
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z53-W539
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ