Different amounts of DNA in each mitochondrion in rice root

• Takanashi Hideki

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Arimura Shin-ichi

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Sakamoto Wataru

  Research Institute for Bioresources, Okayama University

• Tsutsumi Nobuhiro

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

抄録

The DNA content of individual mitochondria in rice root cells was analyzed by fluorescence microscopy. Differences in DNA content were detected between individual mitochondria. Some mitochondria contained no detectable nucleoid (DNA-protein complexes). The percent of mitochondria with DAPI(4',6-Diamidino-2-phenylindole) -stained nucleoids varied over the length of the root (root base, 33%; middle portion of root, 41%; root tip, 91%). The mean amounts of DNA per mitochondrial nucleoid were equivalent to 46.4 kbp in the root base, 52.0 kbp in the middle portion of root and 124.2 kbp in the root tip. The amount of DNA in individual mitochondria and the ratio of mitochondria with visible nucleoids were higher in the root tip than in other parts of the root. The estimated amount of DNA in almost all of the observed mitochondria was smaller than the amount of DNA equivalent to the rice mitochondrial genome size (490 kbp), even in root tip.<br>

収録刊行物

• Genes & Genetic Systems

  Genes & Genetic Systems 81 (3), 215-218, 2006

  日本遺伝学会

キーワード

• DAPI
• DNA
• fluorescence microscopy
• mitochondria
• nucleoid

詳細情報

• CRID: 1390282680449542656
• NII論文ID: 10018212814
• NII書誌ID: AA11077421
• DOI: 10.1266/ggs.81.215
• ISSN: 18805779
• ISSN: 13417568
• NDL書誌ID: 7998019
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7998019
• Web Site: http://www.jstage.jst.go.jp/article/ggs/81/3/81_3_215/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可