ニガウリのがん細胞アポトーシス誘導効果および炎症性サイトカイン産生抑制効果 Bitter Gourd Induces Apoptosis in HL60 Human Leukemia Cells and Suppresses the Production of Inflammatory Cytokine in RAW264.7 Macrophage Like Cells
We previously demonstrated that bitter gourd (<I>Momordica charantia</I> L.) ethanol extract induced apoptosis in HL60 human leukemia cells. To examine the effect of different bitter gourd cultivars on cancer cell growth, we determined the effect of nine bitter gourd cultivars grown in Miyazaki prefecture on the growth of HL60 cells. Although growth inhibitory effect of seed extracts was stronger than that of pericarp or placenta extracts, all the extracts inhibited the growth of HL60 cells at concentrations of 25-200μg/ml. The extracts of pericarp, placenta and seeds of bitter gourds cultivars induced apoptosis in HL60 cells after 24h incubation.<BR>Suppression of inflammatory responses is expected to reduce inflammatory disease and development of cancer. Therefore, we determined the effect of the major bitter gourd cultivar 'Sadowara 3' on the bacterial lipopolysaccharide (LPS)-induced TNFα production from RAW264.7 mouse macrophage-like cells. Placenta extract inhibited the TNFα production induced by LPS ; however, it did not have any effect on the growth of RAW264.7 cells. The bitter gourd placenta is suggested to possess the suppressive effect on inflammatory responses.
- 日本食品科学工学会誌 : Nippon shokuhin kagaku kogaku kaishi = Journal of the Japanese Society for Food Science and Technology
日本食品科学工学会誌 : Nippon shokuhin kagaku kogaku kaishi = Journal of the Japanese Society for Food Science and Technology 53(8), 408-415, 2006-08-15