A One-Enzyme PCR-RFLP Assay for Identification of Six Medically Important Candida Species
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Early identification of <i>Candida</i> isolates to the species level is necessary for effective antifungal therapy, and can also facilitate control of hospital infections. Phenotype-based methods for identifying <i>Candida</i> species are often difficult and time-consuming. Molecular biological techniques provide a useful alternative approach. In the present study, the ITS1-5.8S-ITS2 regions of fungal rRNA genes were amplified with universal primers in 20 standard strains. Digestion of the PCR products with one restriction enzyme, <i>Msp</i>I, allowed discrimination of medically important <i>Candida</i> species, including <i>C. albicans</i>, <i>C. glabrata</i>, <i>C. parapsilosis</i>, <i>C. tropicalis</i>, <i>C. krusei</i>, and <i>C. guilliermondii</i>. Using this method, we successfully identified 137 clinical isolates of <i>Candida</i>. Among them, <i>C. albicans</i> was identified as the most common species, followed by <i>C. parapsilosis</i>, <i>C. tropicalis</i>, <i>C. glabrata</i>, <i>C. krusei</i>, and <i>C. guilliermondii</i>. This method is a simple, rapid, and cost-effective method for differentiation between species that is applicable in clinical laboratories.
- Japanese Journal of Medical Mycology
Japanese Journal of Medical Mycology 47(3), 225-229, 2006-07-31
The Japanese Society for Medical Mycology