Acetylcholine Inhibits the Hypoxia-Induced Reduction of Connexin43 Protein in Rat Cardiomyocytes
-
- Zhang Yanan
- Department of Clinical Laboratory, Kochi Medical School, Japan
-
- Kakinuma Yoshihiko
- Department of Cardiovascular Control, Kochi Medical School, Japan
-
- Ando Motonori
- Department of Cardiovascular Control, Kochi Medical School, Japan
-
- Katare Rajesh G
- Department of Cardiovascular Control, Kochi Medical School, Japan
-
- Yamasaki Fumiyasu
- Department of Clinical Laboratory, Kochi Medical School, Japan
-
- Sugiura Tetsuro
- Department of Clinical Laboratory, Kochi Medical School, Japan
-
- Sato Takayuki
- Department of Cardiovascular Control, Kochi Medical School, Japan
この論文をさがす
抄録
In a recent study, we demonstrated that vagal stimulation increases the survival of rats with myocardial infarction by inhibiting lethal arrhythmia through regulation of connexin43 (Cx43). However, the precise mechanisms for this effect remain to be elucidated. To investigate these mechanisms and the signal transduction for gap junction regulation, we investigated the effect of acetylcholine (ACh), a parasympathetic nerve system neurotransmitter, on the gap junction component Cx43 using H9c2 cells. When cells were subjected to hypoxia, the total Cx43 protein level was decreased. In contrast, pretreatment with ACh inhibited this effect. To investigate the signal transduction, cells were pretreated with <sc>L</sc>-NAME, a nitric oxide synthase inhibitor, followed by ACh and hypoxia. <sc>L</sc>-NAME was found to suppress the ACh effect. However, a NO donor, SNAP, partially inhibited the hypoxia-induced reduction in Cx43. To delineate the mechanisms of the decrease in Cx43 under hypoxia, cells were pretreated with MG132, a proteasome inhibitor. Proteasome inhibition produced a striking recovery of the decrease in the total Cx43 protein level under hypoxia. However, cotreatment with MG132 and ACh did not produce any further increase in the total Cx43 protein level. Functional studies using ACh or okadaic acid, a phosphatase inhibitor, revealed that both reagents inhibited the decrease in the dye transfer induced by hypoxia. These results suggest that ACh is responsible for restoring the decrease in the Cx43 protein level, resulting in functional activation of gap junctions.<br>
収録刊行物
-
- Journal of Pharmacological Sciences
-
Journal of Pharmacological Sciences 101 (3), 214-222, 2006
公益社団法人 日本薬理学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001205177451520
-
- NII論文ID
- 10018236929
-
- NII書誌ID
- AA11806667
-
- COI
- 1:CAS:528:DC%2BD28XnvFalsL4%3D
-
- ISSN
- 13478648
- 13478613
-
- NDL書誌ID
- 7985579
-
- PubMed
- 16829709
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可