The Role of Nitric Oxide in the Electrical Field Stimulation-Induced Contractions of Sphincter of Oddi and Gallbladder Strips in Guinea Pigs

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The aim of this study was to investigate the modulatory role of nitric oxide (NO) in the electrical field stimulation (EFS)-induced contractions of isolated sphincter of Oddi (SO) and gallbladder strips from guinea pigs. EFS was used to activate the intrinsic nerves in SO and gallbladder strips. EFS produced frequency-dependent biphasic contractile responses in the SO strips. A smaller contraction, "on response", occurred during EFS, which was followed by a bigger contraction, "off response". Both responses were completely and irreversibly abolished by tetrodotoxin (TTX) (10<sup>−</sup><sup>6</sup> M). Atropine (10<sup>−</sup><sup>6</sup> M) inhibited the "on response", but not the "off response". EFS produced frequency-dependent monophasic contractile responses in gallbladder strips, which were completely and irreversibly abolished by TTX (10<sup>−</sup><sup>6</sup> M) and atropine (10<sup>−</sup><sup>6</sup> M). A nitric oxide synthase (NOS) inhibitor, <i>N</i><sup>G</sup>-nitro-<sc><font size = "-2">L</font></sc>-arginine (10<sup>−</sup><sup>4</sup> M and 3 × 10<sup>−</sup><sup>4</sup> M, in SO and gallbladder strips, respectively), significantly increased all EFS-induced contractions of SO and gallbladder strips. <sc><font size = "-2">L</font></sc>-Arginine, but not <sc><font size = "-2">D</font></sc>-arginine reversed the effect induced by the NOS inhibitor, at all frequencies, in both strips. These results suggested that NO released from nitrergic nerve endings might play a regulatory role in the cholinergic neurotransmission of guinea pig SO and gallbladder strips. The "off response" in he SO preparations might be a rebound increase that was modulated by the nonadrenergic, noncholinergic inhibitory mediator NO.<br>

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  • Journal of pharmacological sciences  

    Journal of pharmacological sciences 101(3), 240-244, 2006-07-20 

    The Japanese Pharmacological Society

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各種コード

  • NII論文ID(NAID)
    10018237033
  • NII書誌ID(NCID)
    AA11806667
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13478613
  • NDL 記事登録ID
    7985610
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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