Development and Evaluation of a Direct Sandwich Enzyme-Linked Immunosorbent Assay for the Quantification of Guinea-Pig Immunoglobulin E

• Nishimura Makoto

  Dainippon Sumitomo Pharma Co., Ltd., Japan

• Funaoka Hiroyuki

  Dainippon Sumitomo Pharma Co., Ltd., Japan

• Hosoe Hiroaki

  Dainippon Sumitomo Pharma Co., Ltd., Japan

• Ohkaru Yasuhiko

  Dainippon Sumitomo Pharma Co., Ltd., Japan

• Yakuo Ikuhisa

  Dainippon Sumitomo Pharma Co., Ltd., Japan

• Hayakawa Shinobu

  Maruko Pharmaceutical Co., Ltd., Japan

• Ito Koji

  Yugawara Kouseinenkin Hospital, Japan

抄録

The purpose of this study was to develop and evaluate a direct sandwich enzyme-linked immunosorbent assay (ELISA) for the immunoglobulin E (IgE) in serum and plasma from guinea pig using mouse monoclonal antibodies specific for guinea-pig IgE. Mouse monoclonal antibodies were raised against purified IgE protein. The ELISA was performed using a combination of two anti-IgE monoclonal antibodies. One antibody was labeled with horseradish peroxidase (HRP), and the other was coated on polystyrene wells. Purified guinea-pig IgE was used as the standard material. The validity of the ELISA was confirmed by precision, dilution, recovery, and interference tests. The range of detection was 3.1 – 800 ng of IgE mass per mL of serum and plasma. The intra- and inter-assay coefficients of variation were 4.6% and 5.7%, respectively, or less. The recovery test showed variation only between 92.1% and 111.8%, and the anticoagulants showed noninterference with the IgE assay. The mean serum IgE mass concentration in OVA-sensitized guinea pigs was 29438 ng/mL, and it was 48.6 ng/mL in normal guinea pigs. The present ELISA is useful and practical for specific measurement of the guinea-pig IgE, and it is surmised that it would be suitable for use in allergological and pharmacological research.<br>

収録刊行物

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 101 (1), 58-65, 2006

  公益社団法人 日本薬理学会

キーワード

• Immunoglobulin E
• sandwich enzyme-linked immunosorbent assay
• monoclonal antibody
• quantification

詳細情報

• CRID: 1390282680153223040
• NII論文ID: 10018237381
• NII書誌ID: AA11806667
• DOI: 10.1254/jphs.fpj05033x
• ISSN: 13478648
• ISSN: 13478613
• ISSN: http://id.crossref.org/issn/13478613
• NDL書誌ID: 7915751
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7915751
• Web Site: https://search.jamas.or.jp/link/ui/2007004668
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可