Epidermal Growth Factor Induces Vasoconstriction Through the Phosphatidylinositol 3-Kinase-Mediated Mitogen-Activated Protein Kinase Pathway in Hypertensive Rats
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- Kim Junghwan
- Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Korea
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- Lee Chang-Kwon
- Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Korea
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- Park Hyo-Jun
- Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Korea
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- Kim Hyo Jin
- Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Korea
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- So Hyun Ha
- Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Korea
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- Lee Keun Sang
- Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Korea
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- Lee Hwan Myung
- Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Korea
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- Roh Hui Yul
- Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Korea
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- Choi Wahn Soo
- Department of Immunology, College of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Korea
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- Park Tae Kyu
- Department of Biotechnology, College of Biomedical & Health Science, Konkuk University, Korea
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- Kim Bokyung
- Department of Physiology, College of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Korea
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Abstract
We investigated whether increased contractile responsiveness to epidermal growth factor (EGF) is associated with altered activation of mitogen-activated protein kinase (MAPK) in the aortic smooth muscle of deoxycorticosterone acetate (DOCA)-salt hypertensive rats. EGF induced contraction and MAPK activity in aortic smooth muscle strips, which were significantly increased in tissues from the DOCA-salt hypertensive rats compared with those from sham-operated rats. AG1478, PD98059, and LY294002, inhibitors of EGF receptor (EGFR) tyrosine kinase, MAPK/extracellular signal-regulated kinase (ERK) kinase, and phosphatidylinositol 3-kinase (PI3K), respectively, inhibited the contraction and the activity of ERK1/2 that were elevated by EGF. Y27632 and GF109203X, inhibitors of Rho kinase and protein kinase C, respectively, attenuated EGF-induced contraction, with no diminution of ERK1/2 activity. Although EGF also elevated the activity of EGFR tyrosine kinase in both sham-operated and DOCA-salt hypertensive rats, the expression and the magnitude of activation did not differ between strips. These results strongly suggest that EGF induces contraction by the activation of ERK1/2, which is regulated by the PI3K pathway in the aortic smooth muscle of DOCA-salt hypertensive rats.<br>
Journal
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- Journal of Pharmacological Sciences
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Journal of Pharmacological Sciences 101 (2), 135-143, 2006
The Japanese Pharmacological Society
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Keywords
Details 詳細情報について
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- CRID
- 1390282680152864512
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- NII Article ID
- 10018237741
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- NII Book ID
- AA11806667
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- COI
- 1:CAS:528:DC%2BD28XmslWrsb0%3D
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- ISSN
- 13478648
- 13478613
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- NDL BIB ID
- 7963315
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- PubMed
- 16766857
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed