Characterization of Muscarinic Receptor-Mediated Cationic Currents in Longitudinal Smooth Muscle Cells of Mouse Small Intestine

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著者

    • UCHIYAMA Mai
    • Laboratory of Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University
    • HATTORI Mitsunobu
    • Laboratory of Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University
    • NISHIMURA Masakazu
    • Department of Pathogenic Veterinary Science, United Graduate School of Veterinary Science, Gifu University
    • KOMORI Seiichi
    • Laboratory of Pharmacology, Department of Veterinary Medicine, Faculty of Applied Biological Science, Gifu University

抄録

In mouse intestinal smooth muscle cells held at −50 mV, carbachol evoked an atropine-sensitive inward current in the intracellular presence of Cs<sup>+</sup>. The current response consisted of an initial peak followed by a smaller plateau component on which oscillatory currents frequently arose. Results from various experimental procedures indicated that the inward current is a muscarinic receptor-operated cationic current (mI<sub>cat</sub>) sensitive to cytosolic Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) and that the initial peak and oscillatory components are contaminated by Ca<sup>2+</sup>-activated Cl<sup>−</sup> currents. Under conditions of [Ca<sup>2+</sup>]<sub>i</sub> buffered to 100 nM, the mI<sub>cat</sub> response to cumulative carbachol applications was inhibited competitively by an M<sub>2</sub>-selective antagonist but non-competitively by an M<sub>3</sub>-selective one. Also it was severely reduced by pertussis toxin (PTX) treatment or a phospholipase C (PLC) inhibitor. Comparative analysis of mI<sub>cat</sub> in mouse and guinea-pig intestinal myocytes indicated that the underlying channels resemble between those myocytes in agonist sensitivity, current-voltage relationship, and unitary conductance. The results suggest that in mouse intestinal myocytes, mI<sub>cat</sub> arises mainly via an M<sub>2</sub>/M<sub>3</sub> synergistic mechanism involving PTX-sensitive G-proteins and PLC activity in the absence of current modulation by [Ca<sup>2+</sup>]<sub>i</sub> changes, as described for guinea-pig ileal mI<sub>cat</sub>. The channels underlying mI<sub>cat</sub> are also indistinguishable in gating properties between both types of myocytes.<br>

収録刊行物

  • Journal of pharmacological sciences  

    Journal of pharmacological sciences 100(3), 215-226, 2006-03-20 

    The Japanese Pharmacological Society

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各種コード

  • NII論文ID(NAID)
    10018240374
  • NII書誌ID(NCID)
    AA11806667
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13478613
  • NDL 記事登録ID
    7858260
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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