Inhibitory Mechanism of Monensin on High K〔+〕-Induced Contraction in Guniea-Pig Urinary Bladder

• Kaneda Takeharu

  Division of Veterinary Pharmacology, Nippon Veterinary and Animal Science University, Japan

• Takeuchi Mayumi

  Division of Veterinary Pharmacology, Nippon Veterinary and Animal Science University, Japan

• Shimizu Kazumasa

  Division of Veterinary Pharmacology, Nippon Veterinary and Animal Science University, Japan

• Urakawa Norimoto

  Division of Veterinary Pharmacology, Nippon Veterinary and Animal Science University, Japan

• Nakajyo Shinjiro

  Division of Veterinary Pharmacology, Nippon Veterinary and Animal Science University, Japan

• Mochizuki-Kobayashi Mariko

  Division of Veterinary Public Health, Nippon Veterinary and Animal Science University, Japan

• Ueda Fukiko

  Division of Veterinary Public Health, Nippon Veterinary and Animal Science University, Japan

• Hondo Ryo

  Division of Veterinary Public Health, Nippon Veterinary and Animal Science University, Japan

書誌事項

タイトル別名

• Inhibitory Mechanism of Monensin on High K+-Induced Contraction in Guniea-Pig Urinary Bladder

抄録

In this study, we examined the inhibitory mechanism of monensin on high K⁺-induced contraction in guinea-pig urinary bladder. The relaxant effect of monensin (0.001 – 10 μM) was more potent than those of NaCN (100 μM – 1 mM) and forskolin (3 – 10 μM). Monensin (0.1 μM), NaCN (300 μM), or forskolin (10 μM) inhibited high K⁺-induced contraction without decreasing [Ca²⁺]_i level. Monensin and NaCN remarkably decreased creatine phosphate and ATP contents. Monensin and NaCN inhibited high K⁺-induced increases in flavoprotein fluorescence, which is involved in mitochondrial respiration. Forskolin increased cAMP content but monensin did not. Monensin increased Na⁺ content at 10 μM but not at 0.1 μM that induced maximum relaxation. In the α-toxin-permeabilized muscle, forskolin significantly inhibited the Ca²⁺-induced contraction, but monensin did not affect it. These results suggest that the relaxation mechanism of monensin in smooth muscle of urinary bladder may be an inhibition of oxidative metabolism.<br>

収録刊行物

• Journal of Pharmacological Sciences

  Journal of Pharmacological Sciences 100 (2), 133-141, 2006

  公益社団法人 日本薬理学会

キーワード

• monensin
• urinary bladder
• phosphocreatine
• Na⁺ content

詳細情報

• CRID: 1390001205177573504
• NII論文ID: 10018240726
• NII書誌ID: AA11806667
• DOI: 10.1254/jphs.fpj05022x
• ISSN: 13478648
• ISSN: 13478613
• NDL書誌ID: 7821850
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7821850
• Web Site: https://search.jamas.or.jp/link/ui/2006310783
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可