Anti-arthritic Effects of Ephedra sinica STAPF Herb-Acupuncture : Inhibition of Lipopolysaccharide-Induced Inflammation and Adjuvant-Induced Polyarthritis

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著者

    • Yeom Mi-Jung YEOM Mi-Jung
    • Laboratory of Acupuncture & Meridian, Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University
    • Lee Han-Chang LEE Han-Chang
    • Laboratory of Acupuncture & Meridian, Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University
    • Kim Gun-Ho [他] KIM Gun-Ho
    • Laboratory of Acupuncture & Meridian, Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University
    • LEE Hye-Jung
    • Laboratory of Acupuncture & Meridian, Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University
    • SHIM Insop
    • Department of Integrative Medicine, College of Medicine, The Catholic University of Korea
    • OH Seung-Kyu
    • Laboratory of Acupuncture & Meridian, Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University
    • KANG Sung-Keel
    • Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-Hee University
    • HAHM Dae-Hyun
    • Laboratory of Acupuncture & Meridian, Department of Oriental Medical Science, Graduate School of East-West Medical Science, Kyung-Hee University

抄録

Anti-inflammatory and anti-arthritic effects of water distillates of <i>Ephedra sinica</i> S<sc><font size=-2>TAPF</font></sc> (ES), in herb-acupuncture, on the inflammatory responses of arthritis was investigated using phorbol 12-myristate 13-acetate (PMA)/lipopolysaccharide (LPS)-induced human macrophage and adjuvant-induced arthritic rat. The luciferase reporter vectors driven by the tumor necrosis factor (TNF)-α and cyclooxygenase-2 promoters were transiently transfected into U937 cells, which were then differentiated and stimulated by PMA and LPS, respectively, to develop an in vitro anti-inflammation assay system. The luciferase activities, observed in the activated U937 cells, were significantly inhibited by ES herb-acupuncture, compared to those of PD98509 and berberine. To evaluate ES herb-acupuncture as a novel anti-arthritic therapy, a polyarthritic rat model was developed using heat-killed <i>Mycobacterium tuberculosis</i>, and 50 μl of ES distillate was subcutaneously injected into the ST36 acupoint on each knee joint. While the articular indexes of arthritic rats were evidently decreased by ES herb-acupuncture, their body weights did not regain their initial levels. This may be due to the accelerating effects of ES on weight-loss and fat consumption. The mRNA expressions of TNF-α and interleukin (IL)-6 genes, which were closely stimulated in the arthritic rat joints, were found to be restored to the normal levels through the ES treatment. In the case of IL-1β, the recovery was not significant but substantial. The anti-arthritic effect of ES herb-acupuncture was not found in the ES-treated/non-acupoint group. In conclusion, the ES herb-acupuncture into the ST36 acupoint was found to be effective in alleviating the inflammatory response and thus arthritic symptoms in adjuvant-induced arthritic rats.<br>

収録刊行物

  • Journal of pharmacological sciences  

    Journal of pharmacological sciences 100(1), 41-50, 2006-01-20 

    The Japanese Pharmacological Society

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各種コード

  • NII論文ID(NAID)
    10018241074
  • NII書誌ID(NCID)
    AA11806667
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13478613
  • NDL 記事登録ID
    7782977
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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