Detection of Listeria monocytogenes from Food Samples by PCR after IMS-Plating
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For rapid, accurate and sensitive detection of <I>Listeria monocytogenes</I> in food samples, colonies developed on the selective agar (Oxford agar) after immunomagnetic separation (IMS) were subjected to polymerase chain reaction (PCR) assay with the prf A1-2 primer pair. The proposed assay system was shown experimentally to be capable of specifically detecting the bacteria from food samples contaminated at more than 10<SUP>2</SUP> cfu/g. However, the enrichment culture after a short period of 16 h with the appropriate selective broth was needed before IMS-plating, because the bacterial contents in most actual food were as low as less than 10<SUP>2</SUP> cfu/g. However, even if the enrichment cultivation was employed before IMS, L. monocytogenes was detected within 3 days.
- Biocontrol Sci.
Biocontrol Sci. 11(3), 129-134, 2006-09-01
The Society for Antibacterial and Antifungal Agents, Japan