Detection of Listeria monocytogenes from Food Samples by PCR after IMS-Plating

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For rapid, accurate and sensitive detection of <I>Listeria monocytogenes</I> in food samples, colonies developed on the selective agar (Oxford agar) after immunomagnetic separation (IMS) were subjected to polymerase chain reaction (PCR) assay with the prf A1-2 primer pair. The proposed assay system was shown experimentally to be capable of specifically detecting the bacteria from food samples contaminated at more than 10<SUP>2</SUP> cfu/g. However, the enrichment culture after a short period of 16 h with the appropriate selective broth was needed before IMS-plating, because the bacterial contents in most actual food were as low as less than 10<SUP>2</SUP> cfu/g. However, even if the enrichment cultivation was employed before IMS, L. monocytogenes was detected within 3 days.

収録刊行物

  • Biocontrol science  

    Biocontrol science 11(3), 129-134, 2006-09-01 

    The Society for Antibacterial and Antifungal Agents, Japan

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各種コード

  • NII論文ID(NAID)
    10018251794
  • NII書誌ID(NCID)
    AA11169621
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424815
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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