Detection of Listeria monocytogenes from Food Samples by PCR after IMS-Plating

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Abstract

For rapid, accurate and sensitive detection of <I>Listeria monocytogenes</I> in food samples, colonies developed on the selective agar (Oxford agar) after immunomagnetic separation (IMS) were subjected to polymerase chain reaction (PCR) assay with the prf A1-2 primer pair. The proposed assay system was shown experimentally to be capable of specifically detecting the bacteria from food samples contaminated at more than 10<SUP>2</SUP> cfu/g. However, the enrichment culture after a short period of 16 h with the appropriate selective broth was needed before IMS-plating, because the bacterial contents in most actual food were as low as less than 10<SUP>2</SUP> cfu/g. However, even if the enrichment cultivation was employed before IMS, L. monocytogenes was detected within 3 days.

Journal

  • Biocontrol Sci.  

    Biocontrol Sci. 11(3), 129-134, 2006-09-01 

    The Society for Antibacterial and Antifungal Agents, Japan

References:  14

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Cited by:  2

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Codes

  • NII Article ID (NAID)
    10018251794
  • NII NACSIS-CAT ID (NCID)
    AA11169621
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13424815
  • Data Source
    CJP  CJPref  J-STAGE 
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