Detection of Listeria monocytogenes from Food Samples by PCR after IMS-Plating

• UEDA SHIGEKO

  Laboratory of Hygiene, Kagawa Nutrition University

• MARUYAMA TSUTOMU

  Japan Food Hygiene Association

• KUWABARA YOSHIHIRO

  Laboratory of Hygiene, Kagawa Nutrition University

Abstract

For rapid, accurate and sensitive detection of Listeria monocytogenes in food samples, colonies developed on the selective agar (Oxford agar) after immunomagnetic separation (IMS) were subjected to polymerase chain reaction (PCR) assay with the prf A1-2 primer pair. The proposed assay system was shown experimentally to be capable of specifically detecting the bacteria from food samples contaminated at more than 10² cfu/g. However, the enrichment culture after a short period of 16 h with the appropriate selective broth was needed before IMS-plating, because the bacterial contents in most actual food were as low as less than 10² cfu/g. However, even if the enrichment cultivation was employed before IMS, L. monocytogenes was detected within 3 days.

Journal

• Biocontrol Science

  Biocontrol Science 11 (3), 129-134, 2006

  The Society for Antibacterial and Antifungal Agents, Japan

Keywords

• <I>Listeria monocytogenes</I>
• IMS
• PCR
• Food

Details

• CRID: 1390001204464400512
• NII Article ID: 10018251794
• NII Book ID: AA11169621
• DOI: 10.4265/bio.11.129
• ISSN: 18840205
• ISSN: 13424815
• PubMed: 17017131
• Web Site: https://search.jamas.or.jp/link/ui/2007087857
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed