Effect of emetine, a protein synthesis inhibitor, on larval tail resorption in the ascidian Halocynthia roretzi

• Matsumura Kiyotaka

  Fusetani Biofouling Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST) Present Address: Environmental Science Research Laboratory, Central Research Institute of Electric Power Industry

• Nagano Manami

  Fusetani Biofouling Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST)

• Tsukamoto Sachiko

  Fusetani Biofouling Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST) Present Address: Graduate School of Natural Science and Technology, Kanazawa University

• Kato Haruko

  Fusetani Biofouling Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST)

• Fusetani Nobuhiro

  Fusetani Biofouling Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Agency (JST) Present Address: Graduate School of Fisheries Sciences, Hokkaido University

Abstract

Ascidian tadpole larvae settle and metamorphose after a free-swimming period, and one of the typical morphological changes during metamorphosis is tail resorption. Halocynthia roretzi larvae cannot metamorphose for 6hrs after hatching even in the presence of metamorphosis inducers, which suggests that some internal changes occur in the larvae during this period. Larval protein synthesis was examined as one possibility by using a protein synthesis inhibitor, emetine. When tadpole larvae just after hatching were incubated with 5-10μM emetine, tail resorption did not occur. In contrast, larvae that were treated after a free-swimming period of 8 or 24hrs underwent tail resorption, although some were abnormal. These results suggest that protein synthesis during the swimming period is necessary for tail resorption.

Journal

• Sessile Organisms

  Sessile Organisms 23 (2), 43-46, 2006

  THE SESSILE ORGANISMS SOCIETY OF JAPAN

Keywords

• ascidian
• larva
• metamorphosis
• protein synthesis
• emetine

Details

• CRID: 1390282679343304192
• NII Article ID: 130003656227
• NII Article ID: 10018254289
• NII Book ID: AN10563630
• DOI: 10.4282/sosj.23.2_43
• ISSN: 18834701
• ISSN: 13424181
• NDL BIB ID: 8095326
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8095326
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed