タマネギの品種識別用DNAマーカーの開発  [in Japanese] Development of DNA Markers for Identification of Onion Cultivars  [in Japanese]

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Abstract

日本,ニュージーランド,オーストラリア,アメリカ及びタイのタマネギ(<I>Allium cepa</I> L.)20品種を供試試料とし,品種間における遺伝的多様性を解析した.その結果,再現性の高い19個のSTSマーカーを作成した.これらマーカーを用いて各品種24個体における品種内個体差を調査した.葉緑体及びミトコンドリアDNAより作成したマーカーは品種内個体差がほとんどなかったが,その他のマーカーについては品種内個体差が多く確認された.これはタマネギが他殖性植物であることが原因と考えられ,現技術ではタマネギの1個体での品種識別は不可能であることが確認された.しかしながら,品種間において各マーカーのアリール頻度は一定の傾向を示し,これら頻度を2群の比率の差の検定で比較することにより品種を識別できる可能性が示唆された.

The possibility of identification of onion (<I>Allium cepa</I> L.) cultivars by DNA analysis was investigated. The genetic diversity of twenty onion cultivars from Japan, New Zealand, Australia, the USA and Thailand were examined, and 19 sets of sequence-tagged site (STS) primers were designed. Discriminating bands were amplified by each set of STS primers using DNA extracted from 24 onion bulbs of each cultivar. Individual variations in DNA bands in each cultivar were shown, which caused difficulties in discrimination between cultivars in the case of two sets of STS primers which were designed from cytoplasmic DNA (chloroplastic and mitochondrial DNA). However, some individual variations were shown in each cultivar by using the other seventeen sets of STS primers which were designed based on genomic DNA. These results suggested that the identification of onion cultivars using a single onion bulb is impossible. However, because it was shown that the population of allele frequency derived from each cultivar tended to be constant, it may be possible to identify onion cultivars using a significance test to compare allele frequencies of two populations derived from multiple onion bulbs of two varieties.

Journal

  • NIPPON SHOKUHIN KOGYO GAKKAISHI  

    NIPPON SHOKUHIN KOGYO GAKKAISHI 53(9), 498-504, 2006-09-15 

    Japanese Society for Food Science and Technology

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Cited by:  3

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Codes

  • NII Article ID (NAID)
    10018255008
  • NII NACSIS-CAT ID (NCID)
    AN10467499
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    1341027X
  • NDL Article ID
    8048860
  • NDL Source Classification
    ZP34(科学技術--食品工学・工業)
  • NDL Call No.
    Z17-126
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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