Identification of an immunodominant antigen of Mycobacterium leprae and its application for the development of protective measure

• MAKINO Masahiko

  Department of Microbiology, leprosy Research Center, National Institute of Infectious Diseases

Bibliographic Information

Other Title

• 高免疫原性分子の同定と予防法への応用

Abstract

Host defense against Mycobacterium leprae (M. leprae) is chiefly conducted by cellular immunity. The adaptive immunity plays an important role, and T cells are activated through recognition of some immunodominant antigens of M. leprae. A search for an immunodominant antigen was carried out using human peripheral monocytes-derived dendritic cells and M. leprae-derived cell membrane fraction which is the most antigenic fraction of the bacteria, and Major Membrane Protein (MMP)-II was found as one of the immunodoninant antigens. The MMP-II highly stimulated both dendritic cells and macrophages to produce various cytokines. Further, MMP-II was recognized by T cells in vivo in patients infected with M. leprae. Then, we constructed a recombinant BCG secreting MMP-II. The recombinant BCG strongly stimulated both naïve CD4⁺ and naïve CD8⁺ T cells, and seemed to be a useful immunostimulatory agent.

Journal

• JAPANESE JOURNAL OF LEPROSY

  JAPANESE JOURNAL OF LEPROSY 75 (3), 279-283, 2006

  Japanese Leprosy Association

Keywords

• Dendritic cell
• Dominant antigen
• Host defense
• <i>Mycobacterium leprae</i>
• Vaccine

Details

• CRID: 1390001206323387776
• NII Article ID: 10018809746
• NII Article ID: 10018265512
• NII Book ID: AN10559906
• DOI: 10.5025/hansen.75.279
• DOI: 10.1620/tjem.210.279
• ISSN: 1884314X
• ISSN: 13493329
• ISSN: 13423681
• ISSN: 00408727
• Web Site: http://www.jstage.jst.go.jp/article/tjem/210/4/210_4_279/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007038609
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed