Xylanolytic and Alkaliphilic Dietzia sp. Isolated from Larvae of the Japanese Horned Beetle, Trypoxylus dichotomus

この論文にアクセスする

この論文をさがす

著者

    • Urai Makoto [他] URAI Makoto
    • Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
    • AIZAWA Tomoko
    • Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
    • WADA Noriko
    • Laboratory of Applied Biochemistry, Department of Agriculture, Junior College, Nihon University
    • IWABUCHI Noriyuki
    • Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
    • SUNAIRI Michio
    • Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University
    • NAKAJIMA Mutsuyasu
    • Laboratory of Molecular Microbiology, Department of Applied Biological Science, College of Bioresource Sciences, Nihon University

抄録

Larvae of the Japanese horned beetle, <I>Trypoxylus dichotomus septentrionalis</I> (Kono 1931), feed on putrid wood and leaf litter, and are assumed to play important roles in the degradation of polysaccharides produced by broadleaf trees in Japan. Wada <I>et al.</I> showed the presence of strong degrading activities against several polysaccharides, <I>e.g.</I>, β-1, 4-xylan, pectin, and β-1,3-glucan, in the contents of the larval gut and an alkaline pH (pH 10) of the contents of the larval midgut. Xylanolytic strains of bacteria, H05TDL, F09TDL, and W02TDL, were isolated, by use of alkaline media containing 0.5% xylan, from the hindgut and feces of <I>T. dichotomus</I> larvae and putrid wood used for feeding of the larva; and these strains seemed to belong to the genus <I>Dietzia</I> by 16S rDNA analysis. The strain H05TDL showed higher xylanolytic activity at alkaline pH than at neutral pH. The cells of this strain were Gram-positive aerobic rods containing short-chain mycolic acids (33–40 carbon atoms) and arabinose and galactose as the major cell-wall sugars, which also support that the strain belongs to the genus <I>Dietzia</I>. The bacterium grew well between pH 6.8 and 9.6 (optimally at pH 8.3), and produced β-1,4-xylanase, which showed its maximum activity at pH 9.4. In the pH range between 8 and 10, the enzyme activity was 70% of the maximum activity or higher, suggesting that the β-1,4-xylanase may have become adapted to the alkaline lumen of the gut.

収録刊行物

  • 日本放線菌学会誌  

    日本放線菌学会誌 20(2), 49-54, 2006-12-25 

    The Society for Actinomycetes Japan

参考文献:  35件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10018284029
  • NII書誌ID(NCID)
    AN10080091
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09145818
  • NDL 記事登録ID
    8630876
  • NDL 雑誌分類
    ZR5(科学技術--生物学--菌類・微生物)
  • NDL 請求記号
    Z18-1070
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ