ヒトウイルソン病モデル, Long-Evans Cinnamon (LEC) ラット脳細胞における銅の蓄積とDNA損傷の生成 Accumulation of Copper Induces DNA Strand Breaks in Brain Cells of Long-Evans Cinnamon (LEC) Rats, An Animal Model for Human Wilson Disease

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著者

    • 林 正信 HAYASHI Masanobu
    • 酪農学園大学獣医学部放射線獣医学 Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University
    • 布施 清夏 FUSE Sayaka
    • 酪農学園大学獣医学部放射線獣医学 Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University
    • 遠藤 大二 [他] ENDOH Daiji
    • 酪農学園大学獣医学部放射線獣医学 Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University
    • 堀口 昇 HORIGUCHI Noboru
    • 酪農学園大学獣医学部放射線獣医学 Department of Veterinary Radiology, School of Veterinary Medicine, Rakuno Gakuen University
    • 昆 泰寛 KON Yasuhiro
    • 北海道大学獣医学研究科解剖学 Laboratory of Anatomy, Graduate School of Veterinary Medicine, Hokkaido University

抄録

Copper accumulation and induction of DNA strand breaks were investigated in the brain of Long-Evans Cinnamon (LEC) rats, an animal model for human Wilson disease that is a heritable disease of copper accumulation and copper toxicity in the liver, kidney and brain. Copper contents in the brain of LEC rats increased from 20 weeks of age and were approximately 3.5 to 6 folds higher than those in the brain of WKAH rats at 24 weeks of age. Hepatic copper contents in LEC rats increased from 4 to 12 weeks of age in an age-dependent manner, and then decreased from 16 to 20 weeks of age. Thus, we consider that copper accumulated in the liver was released from severely damaged hepatocytes and deposited in the brain, although copper contents in the brain were 1/20-fold lower than those in the liver. We also evaluated the amounts of DNA single-strand breaks (SSBs) in the brain by comet analysis. The proportions of nuclei in the cerebrum and cerebellum without DNA damage decreased, and nuclei with severe DNA damage appeared in LEC rats at 24 weeks of age. The comet scores of cerebrum and cerebellum cells significantly increased in LEC rats and were significantly higher than those in WKAH rats at 24 weeks of age. The results show that SSBs in LEC rat brain cells are induced at a lower concentration of copper than are SSBs in hepatic cells.<br>

収録刊行物

  • Experimental animals  

    Experimental animals 55(5), 419-426, 2006-10-01 

    Japanese Association for Laboratory Animal Science

参考文献:  49件

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各種コード

  • NII論文ID(NAID)
    10018307755
  • NII書誌ID(NCID)
    AA11032321
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13411357
  • NDL 記事登録ID
    8089635
  • NDL 雑誌分類
    ZS7(科学技術--医学)
  • NDL 請求記号
    Z54-H752
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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