流体MEMS中での細胞分離と運動性の定量化 Separation of Cells using a Fluidic MEMS Device and a Quantitative Analysis of Cell Movement

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著者

    • 礒田 隆聡 ISODA Takaaki
    • 北九州市立大学国際環境工学部 環境化学プロセス工学科 Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu
    • 石田 靖明 ISHIDA Yasuaki
    • 北九州市立大学国際環境工学部 環境化学プロセス工学科 Department of Chemical Processes and Environments, Faculty of Environmental Engineering, The University of Kitakyushu

抄録

Fluidic micro electro mechanical system (fluidic MEMS) devices, composed of a micro pump, mixer, valve, reactor, sensor and an electric circuit on a chip, have been widely applied in biotechnology and medical analyses. This study describes the design and fabrication of a fluidic MEMS device that can separate living leukocyte cells from a single droplet of blood (< 1μl). The chip was constructed from two substrate materials sandwiched together to form a gap with an upper hydrophilic (glass) surface and a lower hydrophobic (acrylic resin) surface. A blood sample was flowed into the gap (40μm) between the two substrates driven by the difference in surface tension of the two materials. Leukocyte cells were left adhered to the lower hydrophobic surface, whereas red corpuscles flowed toward the exit of the fluidic MEMS device. The separation rate of the red corpuscles has been achieved to 91 ± 9% in a unit area of 0.1 mm<sup>2</sup>. Further, the change in an area of a living leukocyte cell separated in the chip, was quantitatively analyzed. This study proposes a method for separating and measuring living cells in a fluidic MEMS device.

収録刊行物

  • 電気学会論文誌. E, センサ・マイクロマシン準部門誌 = The transactions of the Institute of Electrical Engineers of Japan. A publication of Sensors and Micromachines Society  

    電気学会論文誌. E, センサ・マイクロマシン準部門誌 = The transactions of the Institute of Electrical Engineers of Japan. A publication of Sensors and Micromachines Society 126(11), 583-589, 2006-11-01 

    The Institute of Electrical Engineers of Japan

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各種コード

  • NII論文ID(NAID)
    10018318788
  • NII書誌ID(NCID)
    AN1052634X
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    13418939
  • NDL 記事登録ID
    8560004
  • NDL 雑誌分類
    ZN31(科学技術--電気工学・電気機械工業)
  • NDL 請求記号
    Z16-B380
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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