Elution Behavior of DNAs in Temperature-Gradient Affinity Chromatography for Single Nucleotide Polymorphism Detection

この論文にアクセスする

この論文をさがす

著者

抄録

A single nucleotide polymorphism (SNP) was detected by the use of affinity chromatography with a single-stranded DNA ligand and temperature gradient elution. Because of the difference in thermostability between the ligand and sample single-stranded DNAs with and without SNPs, they were eluted as a peak with a shoulder by the temperature gradient elution. The sample DNAs with SNPs were detected as low as 0.1 pmol, and the elution behavior of DNAs with and without SNPs in this chromatography was predicted by a numerical calculation method based on a thermodynamic mass transfer model.

収録刊行物

  • Journal of chemical engineering of Japan  

    Journal of chemical engineering of Japan 39(10), 1104-1107, 2006-10-01 

    The Society of Chemical Engineers, Japan

参考文献:  6件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10018326902
  • NII書誌ID(NCID)
    AA00709658
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    00219592
  • NDL 記事登録ID
    8513612
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-R395
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ