Introduction to Protein Crystallography-Inside of a Black Box-(2) Background of Phase Determination: Molecular Replacement Method

  • TAKÉNAKA Akio
    Graduate School of Bioscience and Biotechnology, Tokyo Instite of Technology

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  • タンパク質結晶構造解析入門―ブラックボックスの中身―(2)位相決定法の裏側をのぞいてみよう  分子置換法の原理と実際
  • ニュウモン コウザ タンパクシツ ケッショウ コウゾウ カイセキ ニュウモン ブラックボックス ノ ナカミ 2 イソウ ケッテイホウ ノ ウラガワ オ ノゾイテ ミヨウ ブンシ チカンホウ ノ ゲンリ ト ジッサイ

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Abstract

As an easy technique for phase determination in X-ray analysis of macromolecular crystals, the molecular replacement method is widely used. Available computer programs are highly sophisticated to user friendly so that users can use them without knowledge on the theoretical backgrounds. To develop the technique to more powerful tool, as well as to resolve individual problems in special cases, it is essential to understand the details of the backgrounds. Here the theories of the method with the historical backgrounds, its availability and capability, practical usage and trouble shooting will be described.

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