ビオチンプロテインリガーゼの結晶構造から見えたビオチンの活性化 [in Japanese] Visualization of Biotin Activation Through a Comparison of Crystal Structures of Biotin Protein Ligase [in Japanese]
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Biotin protein ligase (BPL) catalyses the synthesis of an activated form of biotin, biotinyl-5'-AMP, from substrates biotin and ATP followed by biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase. In order to visualize the structural feature of the BPL reaction, crystal structures of BPL from <I>Pyrococcus horikoshii</I> OT3 have been determined in an unliganded form and three liganded forms with biotin, ADP and the reaction intermediate biotinyl-5'-AMP. The exact locations of the ligands and the active site residues allow us to propose a general scheme for the first step of the reaction carried out by BPL.
X-RAYS 48(5), 354-358, 2006-10-31
The Crystallographic Society of Japan