ヒトFEN1-PCNA複合体の結晶構造が示すスイング活性化機構 [in Japanese] Swing Activation of FEN1 Revealed by the Crystal Structure of Human FEN1-PCNA Complex [in Japanese]
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Flap endonuclease-1 (FEN1) removes the RNA primer during Okazaki fragment maturation in lagging strand DNA synthesis. FEN1 also plays a crucial role in the long-patch base excision repair. FEN1 endonuclease activity is markedly stimulated by binding to proliferating cell nuclear antigen (PCNA), which is well known as the'DNA sliding clamp'. We determined the crystal structure of human FEN 1 complexed to PCNA. A hinge region found in FEN1 endows the enzyme with a degree of freedom to swing and direct its core domain toward the flap-DNA substrate.
X-RAYS 48(5), 367-372, 2006-10-31
The Crystallographic Society of Japan