DETECTION OF rpoB MUTATIONS IN RIFAMPICIN-RESISTANT MYCOBACTERIUM KANSASII

• YOSHIDA Shiomi

  Clinical Research Center

• SUZUKI Katsuhiro

  Clinical Research Center

• TSUYUGUCHI Kazunari

  Clinical Research Center

• IWAMOTO Tomotada

  Kobe Institute of Health

• TOMITA Motohisa

  Departmen1t of Clinical Laboratory

• OKADA Masaji

  Clinical Research Center

• SAKATANI Mitsunori

  Department of Respiratory Medicine, National Hospital Organization Kinki-chuo Chest Medical Center

Bibliographic Information

Other Title

• リファンピシン耐性Ｍｙｃｏｂａｃｔｅｒｉｕｍ　ｋａｎｓａｓｉｉにおけるｒｐｏＢ変異の解明
• リファンピシン タイセイ Mycobacterium kansasii ニ オケル rpoB ヘンイ ノ カイメイ

Abstract

[Purpose] To detect rifampicin-resistant mutations in Mycobacter iumkansasii (M. kansasii).<BR>[Methods] We examined t he M. kansasii isolates from sputum of patients at National Hospital Organization Kinkichuo Chest Medical Center from January 1, 2001 to November 30, 2005 using drug-susceptibility testing, and analyzed 69-bp fragment of rpoB gene in rifampicin-resistant strains.<BR>[Results] Three strains from 314 isolates were de termined as rifampicin resistant using drug-susceptibility testing. Those strains showed a rise in minimum inhibitory concentration (MIC), and had the mutations in rpoB gene. These point mutations in codons 513 and 516 were common mutations found in rifampicin-resistant clinical isolates of M. tuberculosis. [Discussion] We verified the association between rpoB gene mutations and rifampicin resistance in M. kansasii.

Journal

• Kekkaku(Tuberculosis)

  Kekkaku(Tuberculosis) 81 (7), 475-479, 2006

  JAPANESE SOCIETY FOR TUBERCULOSIS

Keywords

• <I>Mycobacterium kansasii</I>
• Rifampicin-resistance
• <I>rpoB</I> mutations
• Drug-susceptibility test

Details

• CRID: 1390001204999460480
• NII Article ID: 10018335804
• NII Book ID: AN00073442
• DOI: 10.11400/kekkaku1923.81.475
• ISSN: 18842410
• ISSN: 00229776
• NDL BIB ID: 8037387
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8037387
• Web Site: https://search.jamas.or.jp/link/ui/2006286184
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed