THE ROLE OF SIMULTANEOUS COMBINATION CULTURE WITH SOLID AND LIQUID MEDIA FOR DIAGNOSIS OF PULMONARY TUBERCULOSIS

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  • ITO Kunihiko
    Department of Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association (JATA) Department of Respiratory Medicine, Fukujuji Hospital, JATA
  • AONOAONO Akio
    Department of Clinical Laboratory

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Other Title
  • 肺結核診断時の結核菌検出率から見た液体培地／小川培地併用の意義
  • ハイケッカク シンダンジ ノ ケッカクキン ケンシュツリツ カラ ミタ エキタイバイチ オガワバイチ ヘイヨウ ノ イギ
  • FOR DIAGNOSIS OF PULMONARY TUBERCULOSIS
  • 小川培地併用の意義

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Abstract

[Purpose] To study the usefulness of simultaneous combination culture with egg-based Ogawa medium (1 slant) and MGIT (Mycobacterium growth indicator tube/BectonDickinson) for detection of M. tuberculosis from sputum in sputum-culture proven pulmonary tuberculosis patients. [Object and Method] Retrospective study of s putum-culturepositive pulmonary tuberculosis cases in our hospital (with tuberculosis ward) from Jan. 2002 to Sept. 2003.<BR>[Result] In 1103 sputum culture for th e diagnosis of 370 sputum-culture proven pulmonary tuberculosis cases, 86. 0% were MGIT positive and 79. 5 % were Ogawa positive. Among sputa contaminated on MGIT, 56. 1% (23/41) were positive on Ogawa. Among sputa culture-negative on MGIT, 2. 7% (3/113) were positive on Ogawa. Those 3 sputa were obtained from 3 different patients, and in 2 of them, other sputa were positive on MGIT. Frequency of “ M. tuberculosis strain which can be cultured only by Ogawa ” was supposed to be at maximum 0. 27% (1/370). Of 41 sputa contaminated on MGIT, 15 sputum specimens were re-treated and re-cultured by MGIT. Of these specimens 46. 7% (7/15) were positive on Ogawa, and 73. 3% (11/15) were positive on re-treated MGIT, and the difference was not statistically different.<BR>[Conclusion] From the standpoint of detection of M. tuberculosis from sputum in sputum-culture proven pulmonary tuberculosis patients, simultaneous combination culture of Ogawa medium with MGIT is useful, mainly for risk management of MGIT contamination. But, if re-treated MGIT is done for cases of contaminated on MGIT, simultaneous combination culture of Ogawa medium with MGIT is not necessary.

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