Establishment and Characterization of a Clonal Human Extraskeletal Ewing's Sarcoma Cell Line, EES1

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著者

    • HATORI MASAHITO
    • Department of Orthopaedic Surgery, Tohoku University School of Medicine
    • DOI HIDEYUKI
    • Division of Advanced Surgical Science and Technology, Tohoku University School of Medicine
    • WATANABE MIKA
    • Department of Pathology, Tohoku University School of Medicine
    • HOSAKA MASAMI
    • Department of Orthopaedic Surgery, Tohoku University School of Medicine
    • KOTAJIMA SATOSHI
    • Department of Orthopaedic Surgery, Tohoku University School of Medicine
    • URANO FUMIHIKO
    • Program in Molecular Medicine, University of Massachusetts Medical School
    • HATA JUNICHI
    • National Research Institute for Child Health and Development
    • KOKUBUN SHOICHI
    • Department of Orthopaedic Surgery, Tohoku University School of Medicine

抄録

Ewing's sarcoma, a small round cell sarcoma arising in soft tissue as well as the bone, is one of the most malignant tumors in children and young adults. Few established cell lines of extraskeletal Ewing's sarcoma (EES) have been reported, which made it difficult to examine the biological features of EES. Therefore, we have established a new clonal cell line of EES. We report its morphological characters, results of chromosomal and immunohistochemical analysis. A piece of tumor obtained from the 18-year-old female patient with EES was xenografted in a nude mouse. In vitro subcultured cells were then obtained from this xenograft. A clonal cell line was subsequently established by limiting dilution and designated EES1. EES1 cells had a doubling time of 24 hours. In the xenografted tumor, the cells expressed vimentin, CD99 (MIC2), neuron specific enolase (NSE) and cytokeratin. The original tumor cells also expressed vimentin, CD 99, and NSE, but was negative for cytokeratin. The morphological and immunohistochemical features of this cell line established, except for cytokeratin expression, were consistent with those of the primary tumor. Cytogenetic analysis of EES1 revealed chromosomal translocation of t(11; 12)(q24;ql2). The chimeric fusion of the Ewing's sarcoma gene in band 22q12 with the Friend leukemia virus integration-1 gene in band 11q24 was also demonstrated. Fluorescence in situ hybridization further confirmed the presence of translocation involving the Ewing's sarcoma gene in both the primary tumor and EES1 cells. In conclusion, we have established a human EES cell line EES1, which will provide a useful model for studying various aspects of human EES.

収録刊行物

  • THE TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE  

    THE TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE 210(3), 221-230, 2006-11-01 

    Tohoku University Medical Press

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各種コード

  • NII論文ID(NAID)
    10018349727
  • NII書誌ID(NCID)
    AA00863920
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    00408727
  • データ提供元
    CJP書誌  J-STAGE 
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