Enhancement of MMP-9 Activity in THP-1 Cells by 7-ketocholesterol and Its Suppression by the HMG-CoA Reductase Inhibitor Fluvastatin
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- Ozaki Hiroshi
- Department of Internal Medicine, Sakura Hospital, Toho University School of Medicine, Chiba, Japan.
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- Miyashita Yoh
- Department of Internal Medicine, Sakura Hospital, Toho University School of Medicine, Chiba, Japan.
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- Watanabe Hitoshi
- Clinical Laboratory Medicine, Sakura Hospital, Toho University School of Medicine, Chiba, Japan.
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- Shirai Kohji
- Department of Internal Medicine, Sakura Hospital, Toho University School of Medicine, Chiba, Japan.
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Abstract
We investigated the effect of 7-ketocholesterol (7-KCHO) on the activity of matrix metalloproteinase (MMP-9) in human monocytic THP-1 cells, and the inhibition of this effect by fluvastatin. In cells incubated with 7-KCHO or cholesterol, the activity of MMP-9 was enhanced, accompanying an increase in the secretion of MMP-9 proenzyme (pro-MMP-9). However, the activity of MMP-9 and the amount of pro-MMP-9 were significantly greater following incubation with 7-KCHO than cholesterol. Neither 7-KCHO nor cholesterol influenced the amount of tissue inhibitor of metalloproteinase (TIMP)-1 secreted by THP-1 cells. When fluvastatin was added to the cells, the MMP-9 activity stimulated by 7-KCHO or cholesterol decreased significantly, accompanying a decrease in the secretion of pro-MMP-9 and TIMP-1. The inhibition of pro-MMP-9 secretion by fluvastatin was stronger in the cells incubated with 7-KCHO than with cholesterol. These results suggest that 7-KCHO activates macrophage and enhances MMP-9 activity, and its effects may be inhibited by fluvastatin.
Journal
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- Journal of Atherosclerosis and Thrombosis
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Journal of Atherosclerosis and Thrombosis 12 (6), 308-314, 2005
Japan Atherosclerosis Society
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Details 詳細情報について
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- CRID
- 1390001204431391872
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- NII Article ID
- 130004444155
- 10018365077
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- NII Book ID
- AA11018976
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- COI
- 1:CAS:528:DC%2BD28XnvVyrsL0%3D
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- ISSN
- 18803873
- 13403478
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed