Analysis of Genes Dominantly Expressed in Rat Cerebral Endothelial Cells Using Suppression Subtractive Hybridization
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- Shibata Takahisa
- Department of Clinical Cell Biology, Chiba University Graduate School of Medicine, Chiba, Japan.
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- Misawa Norihiko
- Marine Biotechnology Institute, Iwate, Japan.
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- Takeo Chikari
- Department of Clinical Cell Biology, Chiba University Graduate School of Medicine, Chiba, Japan.
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- Saeki Naokatsu
- Department of Neurosurgery, Chiba University Graduate School of Medicine, Chiba, Japan.
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- Saito Yasushi
- Department of Clinical Cell Biology, Chiba University Graduate School of Medicine, Chiba, Japan.
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- Tatsuno Ichiro
- Department of Clinical Cell Biology, Chiba University Graduate School of Medicine, Chiba, Japan.
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抄録
In mammals, a fully developed, highly branched vascular system specialized for each particular organ or tissue is essential for obtaining metabolic nutrients supply. The formation of a blood-brain barrier that protects against environmental insults is a distinguishing feature of the brain’s vascular system. Since this is accomplished by cerebral endothelial cells (CECs), we analyzed the genes specifically and/or dominantly expressed in rat CECs using Suppression Subtractive Hybridization (SSH). We found 39 genes specifically and/or dominantly expressed in CECs. 24 genes of known function (thrombospondin-2, vimentin, etc.), 13 genes of known sequence but unknown function including 7 of ESTs (SNERG1, rat GPCR, etc.), and 2 novel genes. The physiological significance of these genes in CECs has been under investigation. SSH is useful for identifying genes regulated in an organ-specific manner in cells such as CECs to obtain clarification of their physiological roles.
収録刊行物
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- Journal of Atherosclerosis and Thrombosis
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Journal of Atherosclerosis and Thrombosis 12 (6), 330-337, 2005
一般社団法人 日本動脈硬化学会
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詳細情報
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- CRID
- 1390282679408096128
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- NII論文ID
- 130004444158
- 10018365173
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- NII書誌ID
- AA11018976
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- ISSN
- 18803873
- 13403478
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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- 抄録ライセンスフラグ
- 使用不可