Analysis of Genes Dominantly Expressed in Rat Cerebral Endothelial Cells Using Suppression Subtractive Hybridization

  • Shibata Takahisa
    Department of Clinical Cell Biology, Chiba University Graduate School of Medicine, Chiba, Japan.
  • Misawa Norihiko
    Marine Biotechnology Institute, Iwate, Japan.
  • Takeo Chikari
    Department of Clinical Cell Biology, Chiba University Graduate School of Medicine, Chiba, Japan.
  • Saeki Naokatsu
    Department of Neurosurgery, Chiba University Graduate School of Medicine, Chiba, Japan.
  • Saito Yasushi
    Department of Clinical Cell Biology, Chiba University Graduate School of Medicine, Chiba, Japan.
  • Tatsuno Ichiro
    Department of Clinical Cell Biology, Chiba University Graduate School of Medicine, Chiba, Japan.

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In mammals, a fully developed, highly branched vascular system specialized for each particular organ or tissue is essential for obtaining metabolic nutrients supply. The formation of a blood-brain barrier that protects against environmental insults is a distinguishing feature of the brain’s vascular system. Since this is accomplished by cerebral endothelial cells (CECs), we analyzed the genes specifically and/or dominantly expressed in rat CECs using Suppression Subtractive Hybridization (SSH). We found 39 genes specifically and/or dominantly expressed in CECs. 24 genes of known function (thrombospondin-2, vimentin, etc.), 13 genes of known sequence but unknown function including 7 of ESTs (SNERG1, rat GPCR, etc.), and 2 novel genes. The physiological significance of these genes in CECs has been under investigation. SSH is useful for identifying genes regulated in an organ-specific manner in cells such as CECs to obtain clarification of their physiological roles.

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