Apoptosis Induced by 7-Ketocholesterol is Enhanced in Smooth Muscle Cells Derived from OLETF Rats

• Miyashita Yoh

  Center of Diabetes, Endocrine and Metabolism, Sakura Hospital, Toho University School of Medicine, Chiba, Japan.

• Koide Nobukiyo

  Center of Diabetes, Endocrine and Metabolism, Sakura Hospital, Toho University School of Medicine, Chiba, Japan.

• Oyama Tomokazu

  Center of Diabetes, Endocrine and Metabolism, Sakura Hospital, Toho University School of Medicine, Chiba, Japan.

• Itoh Yoshiaki

  Center of Diabetes, Endocrine and Metabolism, Sakura Hospital, Toho University School of Medicine, Chiba, Japan.

• Shirai Kohji

  Center of Diabetes, Endocrine and Metabolism, Sakura Hospital, Toho University School of Medicine, Chiba, Japan.

抄録

To clarify whether an increased proliferative potential of vascular smooth muscle cells (SMC) under diabetic conditions augments the susceptibility of the cells to 7-ketocholesterol-induced apoptosis, we investigated the difference in sensitivity to 7-ketocholesterol between SMC obtained from diabetic Otsuka Long-Evans Tokushima fatty (OLETF) rats and the control Long-Evans Tokushima Otsuka (LETO) rats. The outgrowth rate from aortic wall explants and cell proliferation were higher in SMC derived from OLETF rats (OLETF-derived SMC) compared to those from LETO rats (LETO-derived SMC). When 7-ketocholesterol was added to SMC, the amount of fragmented DNA increased significantly in OLETF-derived compared to LETO-derived SMC. The amount of fragmented DNA induced by 7-ketocholeaterol decreased significantly in both OLETF- and LETO-derived SMC when they were incubated without fetal bovine serum. By adding PDGF-BB to LETO-derived SMC, the amount of fragmented DNA induced by 7-ketocholesterol increased significantly. These results suggest that apoptosis of SMC induced by 7-ketocholesterol may be accelerated when SMC acquire a high proliferative potential by prolonged exposure to diabetic condition.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ　ａｎｄ　Ｔｈｒｏｍｂｏｓｉｓ

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｔｈｅｒｏｓｃｌｅｒｏｓｉｓ　ａｎｄ　Ｔｈｒｏｍｂｏｓｉｓ 12 (2), 92-97, 2005

  一般社団法人 日本動脈硬化学会

キーワード

• 7-Ketocholesterol
• Plaque rupture
• Apoptosis
• OLETF rat

詳細情報

• CRID: 1390001204431944832
• NII論文ID: 130004444130
• NII論文ID: 10018366047
• NII書誌ID: AA11018976
• DOI: 10.5551/jat.12.92
• ISSN: 18803873
• ISSN: 13403478
• PubMed: 15942119
• Web Site: https://www.jstage.jst.go.jp/article/jat/12/2/12_2_92/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2006088343
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可