Tolerogen-Producing Cells in Allogeneic Bone Marrow Chimeras Established with Spontaneously Leukemia-Prone Mice
Access this Article
Search this Article
Using SL/Kh mice and AKR/J mice, which are animal models for spontaneous pre-B-cell leukemia and thymic lymphoma, respectively, we studied the protective influence of allogeneic bone marrow transplantation (BMT) and the induction of tolerance to Mls-1<sup>a</sup>, a host antigen. When BM cells from allogeneic C57BL/6 mice were used to reconstitute self-tolerance SL/Kh mice, these [B6→SL] chimeric mice survived for a longer time than non-treated SL or [SL→SL] syngeneic chimeras. These findings are compatible with results previously obtained for [B6→AKR] chimeras. In [B10. D2→SL] and [B10. D2→AKR] chimeras, Vβ6<sup>+</sup> T-cells reactive to Mls-1<sup>a</sup> were eliminated 5 weeks after BMT. On the other hand, minor graft versus host reaction (GVHR) abrogated the clonal elimination of Vβ6<sup>+</sup> T-cells in both [B10. D2→SL] and [B10. D2→AKR] chimeras. The cause of this abrogation was attributed to the early disappearance of Mls-1<sup>a</sup>-producing host T-cells in the GVHR chimeras. The cells responsible for the Mls-1<sup>a</sup> production were revealed to be mainly CD8<sup>+</sup> CD44<sup>+</sup> T-cells, by <i>in vitro</i> mixed lymphocyte reaction (MLR) and <i>in vivo</i> tolerance induction. The present findings indicate that host CD8<sup>+</sup> CD44<sup>+</sup> T-cells constitute the major source of Mls-1<sup>a</sup> antigens in the [Mls-1<sup>b</sup>→Mls-1<sup>a</sup>] BM chimera system.
- The journal of the Japanese Society of Lymphoreticular Tissue research
The journal of the Japanese Society of Lymphoreticular Tissue research 41(1), 61-68, 2001-05-01
The Japanese Society for Lymphoreticular Tissue Research