遺伝子発現解析と画像処理によるリーフレタス葉伸長成長の解析 Analysis of Leaf Elongation in Lettuce using Gene Expression and Image Processing

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Gibberellin (GA) are plant hormones play important roles in plant development. GA biosynthetic pathway and the genes encoding most of the GA biosynthetic enzymes have been characterized. GA 3β-hydroxylase (Ls3h1) is an enzyme that catalyzes the late steps in the formation of active GAs, and is a potential control point in the regulation of GA biosynthesis by light. Red light promotes GA<sub>1</sub> synthesis in lettuce seeds by inducing Ls3h1 gene expression via phytochrome action. Endogenous GA<sub>1</sub> content, the main bioactive GA in lettuce, increases after red-light treatment.<BR>The final goal of this research is to develop a new strategy for environmental control of green houses in lettuce production.<BR>We investigated the influence of light irradiation on Ls3h1 gene expression and leaf elongation in leaf lettuce (<I>Lactuca sativa</I> L. cv. "Green wave"). <I>Ls3h1</I> gene expression was analyzed by real time RT-PCR. Leaf elongation was measured using an image analysis system that consisted of an infrared camera and an infrared lamp to capture plant images every 10 minutes for 2 days.<BR>Image analysis indicated that rate of leaf elongation was increased at 30minutes after starting the light period, and uniconazol inhibited this elongation. Real time RT-PCR analysis showed that <I>Ls3h1</I> gene expression was increased at 5 minutes after starting light period, and that this gene expression was maintained.

収録刊行物

  • 植物環境工学  

    植物環境工学 18(4), 271-276, 2006-12-01 

    日本植物工場学会

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各種コード

  • NII論文ID(NAID)
    10018381982
  • NII書誌ID(NCID)
    AA12010914
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    18802028
  • NDL 記事登録ID
    8596068
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z18-3397
  • データ提供元
    CJP書誌  NDL  IR  J-STAGE 
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