遺伝子発現解析と画像処理によるリーフレタス葉伸長成長の解析 [in Japanese] Analysis of Leaf Elongation in Lettuce using Gene Expression and Image Processing [in Japanese]
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Gibberellin (GA) are plant hormones play important roles in plant development. GA biosynthetic pathway and the genes encoding most of the GA biosynthetic enzymes have been characterized. GA 3β-hydroxylase (Ls3h1) is an enzyme that catalyzes the late steps in the formation of active GAs, and is a potential control point in the regulation of GA biosynthesis by light. Red light promotes GA<sub>1</sub> synthesis in lettuce seeds by inducing Ls3h1 gene expression via phytochrome action. Endogenous GA<sub>1</sub> content, the main bioactive GA in lettuce, increases after red-light treatment.<BR>The final goal of this research is to develop a new strategy for environmental control of green houses in lettuce production.<BR>We investigated the influence of light irradiation on Ls3h1 gene expression and leaf elongation in leaf lettuce (<I>Lactuca sativa</I> L. cv. "Green wave"). <I>Ls3h1</I> gene expression was analyzed by real time RT-PCR. Leaf elongation was measured using an image analysis system that consisted of an infrared camera and an infrared lamp to capture plant images every 10 minutes for 2 days.<BR>Image analysis indicated that rate of leaf elongation was increased at 30minutes after starting the light period, and uniconazol inhibited this elongation. Real time RT-PCR analysis showed that <I>Ls3h1</I> gene expression was increased at 5 minutes after starting light period, and that this gene expression was maintained.
- Shokubutsu Kojo Gakkaishi
Shokubutsu Kojo Gakkaishi 18(4), 271-276, 2006-12-01
Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists