USEFULNESS OF VARIABLE NUMBERS OF TANDEM REPEATS TYPING IN CLINICAL STRAINS OF MYCOBACTERIUM AVIUM

DOI Web Site 5 Citations 19 References
  • MORIYAMA Makoto
    Meijo University Gradua 1te School Pharmaceutical Sciences Postgraduate Course Department of Pulmonary Medicine, National Hospittal Organization (NHO) Higashinagoya National Hospital
  • OGAWA Kenji
    Department of Pulmonary Medicine, National Hospittal Organization (NHO) Higashinagoya National Hospital
  • NISHIMORI Kei
    Department of Pulmonary Medicine, National Hospi tal for Geriatric Medicine
  • UCHIYA Keiichi
    Meijo University Gradua 1te School Pharmaceutical Sciences Postgraduate Course
  • ITO Tetsuya
    National Institute of Animal Health
  • YAGI Tetsuya
    国立長寿 医療センター病院呼吸器科
  • NAKASHIMA Ikkou
    国立長寿 医療センター病院呼吸器科
  • NAKAGAWA Taku
    Department of Pulmonary Medicine, National Hospittal Organization (NHO) Higashinagoya National Hospital
  • TARUMI Osamu
    Department of Pulmonary Medicine, National Hospittal Organization (NHO) Higashinagoya National Hospital
  • NIKAI Toshiaki
    Meijo University Gradua 1te School Pharmaceutical Sciences Postgraduate Course

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  • 臨床由来Ｍｙｃｏｂａｃｔｅｒｉｕｍ　ａｖｉｕｍにおけるＶａｒｉａｂｌｅ　Ｎｕｍｂｅｒｓ　ｏｆ　Ｔａｎｄｅｍ　Ｒｅｐｅａｔｓ型別解析法の有用性の検討
  • リンショウ ユライ Mycobacterium avium ニ オケル Variable Numbers of Tandem Repeats カタベツ カイセキホウ ノ ユウヨウセイ ノ ケントウ

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Abstract

[Objectives] We evaluated the usefulness of Variable Numbers of Tandem Repeats (VNTR) analysis, which was recently reported as a new typing method of Mycobacterium avium strains of animal origin, for strain differentiation of clinical isolates of M. avium in comparison with the standard ISl245-RFLP typing method. In addition, forty M. avium isolates recovered from sputum samples of same patient in different times were analyzed with VNTR typing method.<BR>[Sub jects and Methods] The subjects were twenty-four clinical isolates of M. avium stocked at Higashi Nagoya National Hospital and discriminatory power was evaluated with Hunter Gaston Discriminatory Index (HGDI). Furthermore, forty M. avium isolates recovered from sputum samples of one patient obtained at four different times were analyzed by using this VNTR typing method.<BR>[Results] VNTR typing sh owed better discriminatory power for twenty-four clinical isolates than ISl245-RFLP method (HGDI: 0. 975 vs 0. 866). In the second study, pol yclonal infection of four genotype strains with different allele profiles were detected. The ratio of mixture of the four different genotype strains varied during clinical course.<BR>[Conclusion] We considered tha t VNTR typing method was very useful for discriminatory examination of M. avium.

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