Epitope Screening by Use of a Random Peptide-Displayed Phage Library and Polyclonal Antibody-Coupled Liposomes

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Linear epitopes of sperm whale myoglobin for a rabbit polyclonal anti-myoglobin antibody were determined from a random-peptide displayed phage library by biopanning using antibody-coupled liposomes (AB-MLVs). Two kinds of positive phages displaying peptides (RNFGADAQ and YDLDIGAD) homological to the myoglobin sequence were obtained after 4 rounds of biopanning. Binding affinities of antibodies, which were fractionated from the anti-myoglobin antibody by specificity to both the peptides, were higher than that of an antibody fraction specific to the peptide that was screened by biopanning using an antibody-coated polystyrene tube (AB-Tube). The epitope identified in this study, N(D)FGADAQ has not been reported as an epitope of the rabbit anti-myoglobin antibody. These results suggest that biopanning using AB-MLVs shows much higher selectivity to recover phages displaying peptides with high affinity and specificity than that using the AB-Tube.

収録刊行物

  • Journal of chemical engineering of Japan  

    Journal of chemical engineering of Japan 39(11), 1195-1199, 2006-11-01 

    The Society of Chemical Engineers, Japan

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各種コード

  • NII論文ID(NAID)
    10018401719
  • NII書誌ID(NCID)
    AA00709658
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    00219592
  • NDL 記事登録ID
    8553605
  • NDL 雑誌分類
    ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-R395
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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