Determination of Eight Organic Acids in Sake and Wine by Suppressed Ion Chromatography with Triacontyl Groups (C30) as a Stationary Phase

• KITAMI Hideaki

  Sougou Kankyou Bunseki Co., Ltd.

• ISHIHARA Yoshimi

  School of Science, Tokai University

Abstract

A suppressed ion method for the determination method of eight organic acids (formic, malonic, lactic, acetic, malefic, citric, fumaric, and propionic acid) has been studied for reversed-phase high-performance liquid chromatography (RP-HPLC) with a new triacontyl groups (C₃₀) column, coupled with an ultraviolet detector (UV) at 210 nm. A 50 mM ammonium dihydrogenphosphate (NH₄H₂PO₄) aqueous solution (pH 2.0) containing phosphoric acid (H₃PO₄) was used as an optimal mobile phase for the separation of eight organic acids. A good linearity of the calibration curve was obtained in the concentration range of 5 mgl^-1 to 100 mgl^-1 (|r| =0.9999-0.9994) . The detection limit based on S/N=3 were 3.8 mgl^-1 for formic acid, 3.5 mgl^-1 for malonic acid, 3.1 mgl^-1 for lactic acid, 3.4 mgl^-1 for acetic acid, 1.1 mgl^-1 for maleic acid, 3.4 mgl^-1 for citric acid, 3.3 mgl^-1 for fumaric acid, and 3.6 mgl^-1 for propionic acid. This analytical method was successfully applied to the determination of eight organic acids in sake and wine.

Journal

• Journal of Environmental Chemistry

  Journal of Environmental Chemistry 16 (4), 691-696, 2006

  Japan Society for Environmental Chemistry

Keywords

• triacontyl groups (C<SUB>30</SUB>)
• organic acid
• sake
• wine
• suppressed ion chromatography

Details

• CRID: 1390282679347532544
• NII Article ID: 10018449973
• NII Book ID: AN10547099
• DOI: 10.5985/jec.16.691
• ISSN: 18825818
• ISSN: 09172408
• NDL BIB ID: 8634257
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8634257
• Web Site: http://www.jstage.jst.go.jp/article/jec1991/16/4/16_4_691/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed