THE ROLE OF RAD9 IN HEAD AND NECK CANCER

Bibliographic Information

Other Title
  • P53/P21経路におけるRad9の役割と頭頸部癌への関与

Search this article

Abstract

In response to replication perturbation or damage of DNA, cell-cycle checkpoint is activated, which leads to mutually exclusive, two consequences: cell cycle arrest and repair of damaged DNA, or induction of apoptosis. Although previous studies have suggested that a checkpoint molecule, human Rad9, is involved in the process, the exact mechanism remains to be investigated. Here, we studied the role of Rad9 in DNA damage response through the tumor suppressor p53 pathway. Rad9 bound specifically to a p53-consensus DNA-binding sequence in the P21WAF1 promoter, which increased after exposure to UV. The immunoprecipitation and in vitro binding assay indicated p53 associated with Rad9 regardless of phosphorylation status. Taken together with the assessment of immunohistochemistry showing overexpression of Rad9 in head and neck squamous cell carcinoma cell line, the present study demonstrates overexpression of Rad9 leads to dysregulation of checkpoint response after DNA damage occurs in head and neck cancer.

Journal

  • Toukeibu Gan

    Toukeibu Gan 32 (4), 417-422, 2006

    Japan Society for Head and Neck Cancer

Citations (1)*help

See more

References(26)*help

See more

Details

Report a problem

Back to top