Severe Insulin Resistance and Moderate Glomerulosclerosis in a Minipig Model Induced by High-Fat/High-Sucrose/High-Cholesterol Diet

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著者

    • Liu Yi LIU Yi
    • Institute of Cardiovascular Research, School of Life Sciences and Technology
    • LI Qinkai
    • Institute of Cardiovascular Research, School of Life Sciences and Technology
    • CAI Manbo
    • Institute of Cardiovascular Research, School of Life Sciences and Technology
    • ZHANG Chi
    • Institute of Cardiovascular Research, School of Life Sciences and Technology
    • XIAO Junxia
    • Institute of Cardiovascular Research, School of Life Sciences and Technology
    • HOU Hongjie
    • Institute of Cardiovascular Research, School of Life Sciences and Technology
    • LI Hongguang
    • Institute of Cardiovascular Research, School of Life Sciences and Technology
    • ZU Xiuhong
    • Institute of Cardiovascular Research, School of Life Sciences and Technology

抄録

To develop a minipig model of type 2 diabetes that simulates the common manifestations of the metabolic abnormalities and resembles the kidney pathology of type 2 diabetes in the human population, male Chinese Bama minipigs were divided into 2 groups (5 in each) and fed with a control diet (CD) or high-fat/ high-sucrose/ high-cholesterol diet (HFSCD) for 5 months. The biochemical parameters of blood and urine, and the oral glucose tolerance test were monitored after the feeding program. The insulin resistance was estimated by the HOMA-IR index and the glucose elimination constant (K<sub>G</sub>), and beta-cell function by the HOMA-beta index and the acute insulin response (AIR). Glomerulosclerosis index (GSI) was semi-quantitated by the degree of glomerular lesions in kidney sections stained with Masson trichrome. Extracellular matrix deposition in the kidney was examined by the protein expression of type IV collagen, connective tissue growth factor (CTGF) and matrix metalloproteinases 2 (MMP-2) using immunohistochemistry. Feeding HFSCD to minipigs markedly caused hyperglycaemia, hyperinsulinaemia and dyslipidaemia. HOMA-IR was significantly increased while HOMA-beta, AIR and K<sub>G</sub> were obviously decreased in the HFSCD group compared with control group. Microalbuminuria, glucosuria and moderate glomerulosclerosis were exhibited in HFSCD-fed minipigs. The expression of type IV collagen and CTGF was elevated whereas that of MMP-2 was reduced in the kidneys of HFSCD group compared with the CD group. We concluded that feeding HFSCD to Chinese Bama minipigs for 5 months can induce humanoid type 2 diabetes and early-stage diabetic nephropathy, and accelerate extracellular matrix deposition and glomerulosclerosis.<br>

収録刊行物

  • Experimental animals  

    Experimental animals 56(1), 11-20, 2007-01-01 

    Japanese Association for Laboratory Animal Science

参考文献:  20件

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各種コード

  • NII論文ID(NAID)
    10018453361
  • NII書誌ID(NCID)
    AA11032321
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13411357
  • NDL 記事登録ID
    8597925
  • NDL 雑誌分類
    ZS7(科学技術--医学)
  • NDL 請求記号
    Z54-H752
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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